Anti-DRP1 Antibody (CAB17069)[KO Validated]

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SKU:
CAB17069
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-DRP1 Antibody [KO Validated]
Antibody SKU:CAB17069
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human DRP1
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:mouse brain, mouse heart, rat brain, A-549
Immunogen:Recombinant protein of human DRP1
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:10059
Uniprot:O00429
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Golgi apparatus, Membrane, Mitochondrion outer membrane, Peripheral membrane protein, Peroxisome, clathrin-coated pit, cytosol, secretory vesicle, synaptic vesicle membrane
Calculated MW:60kDa/78kDa/79kDa/80kDa/81kDa/82kDa/83kDa
Observed MW:82kDa
Synonyms:DNM1L, DLP1, DRP1, DVLP, DYMPLE, EMPF, EMPF1, HDYNIV
Background:This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:DRP1: Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis- dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport. Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule. Can self-assemble in multimeric ring-like structures. Interacts with BCL2L1; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters. Interacts with FIS1. Interacts with GSK3B and MARCH5. Interacts (via the GTPase and B domains) with UBE2I; the interaction promotes sumoylation of DNM1L, mainly in ite B domain. Interacts with PPP3CA; the interaction dephosphorylates DNM1L and regulates its transition to mitochondria. Interacts witn MID49 and MID51. Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons. Belongs to the dynamin family. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Endoplasmic reticulum; Mitochondrial; Microtubule-binding; EC 3.6.5.5; Apoptosis; Hydrolase; Motor

Chromosomal Location of Human Ortholog: 12p11.21

Cellular Component: Golgi apparatus; microtubule; protein complex; intracellular membrane-bound organelle; synaptic vesicle membrane; mitochondrion; coated pit; cytosol; mitochondrial outer membrane; membrane; perinuclear region of cytoplasm; cytoplasm; peroxisome; cell junction

Molecular Function:GTPase activity; identical protein binding; protein binding; protein homodimerization activity; GTP binding; ubiquitin protein ligase binding; lipid binding

Biological Process: peroxisome fission; mitochondrial fission; release of cytochrome c from mitochondria; positive regulation of protein secretion; positive regulation of apoptosis; apoptosis; metabolic process; regulation of protein oligomerization; endocytosis; mitochondrial fragmentation during apoptosis; cell structure disassembly during apoptosis; protein homotetramerization

Disease: Encephalopathy, Lethal, Due To Defective Mitochondrial And Peroxisomal Fission

NCBI Summary:This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]
UniProt Code:O00429
NCBI GenInfo Identifier:125987821
NCBI Gene ID:10059
NCBI Accession:O00429.2
UniProt Secondary Accession:O00429,O14541, O60709, Q59GN9, Q7L6B3, Q8TBT7, Q9BWM1 Q9Y5J2, A8K4X9, B4DGC9, B4DSU8, J3KPI2,
UniProt Related Accession:O00429
Molecular Weight:736
NCBI Full Name:Dynamin-1-like protein
NCBI Synonym Full Names:dynamin 1-like
NCBI Official Symbol:DNM1L
NCBI Official Synonym Symbols:DLP1; DRP1; DVLP; EMPF; DYMPLE; HDYNIV
NCBI Protein Information:dynamin-1-like protein; dynamin-like protein 4; dynamin-like protein IV; Dnm1p/Vps1p-like protein; dynamin-related protein 1; dynamin family member proline-rich carboxyl-terminal domain less
UniProt Protein Name:Dynamin-1-like protein
UniProt Synonym Protein Names:Dnm1p/Vps1p-like protein; DVLP; Dynamin family member proline-rich carboxyl-terminal domain less; Dymple; Dynamin-like protein; Dynamin-like protein 4; Dynamin-like protein IV; HdynIV; Dynamin-related protein 1
Protein Family:Dynamin-1-like protein
UniProt Gene Name:DNM1L
UniProt Entry Name:DNM1L_HUMAN
Anti-DRP1 Antibody [KO Validated] (CAB17069)Western blot analysis of extracts of various cell lines, using DRP1 antibody (CAB17069) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-DRP1 Antibody [KO Validated] (CAB17069)Western blot analysis of extracts from normal (control) and DRP1 Rabbit pAb knockout (KO) A-549 cells, using DRP1 Rabbit pAb antibody (CAB17069) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-DRP1 Antibody [KO Validated] (CAB17069)Immunofluorescence analysis of C6 cells using DRP1 antibody (CAB17069) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-DRP1 Antibody [KO Validated] (CAB17069)Immunofluorescence analysis of U2OS cells using DRP1 antibody (CAB17069) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-DRP1 Antibody [KO Validated] (CAB17069)Confocal immunofluorescence analysis of U-2 OS cells using DRP1 Polyclonal Antibody (CAB17069) at dilution of 1:200. Blue: DAPI for nuclear staining.

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