Anti-DOK1 Antibody (CAB5687)
- SKU:
- CAB5687
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-DOK1 Antibody |
| Antibody SKU: | CAB5687 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human DOK1 (NP_001372.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, U-937, Jurkat, Mouse lung, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human DOK1 (NP_001372.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDGA VMEG PLFL QSQR FGTK RWRK TWAV LYPA SPHG VARL EFFD HKGS SSGG GRGS SRRL DCKV IRLA ECVS VAPV TVET PPEP GATA FRLD TAQR SHLL AADA PSSA AWVQ TLCR NAFP KGSW TLAP TDNP PKLS ALEM LENS LYSP TWEG SQFW VTVQ RTEA AERC GLHG SYVL RVEA ERLT LLTV GAQS QILE PLLS WPYT LLRR YGRD KVMF SFEA GRRC PSGP GTFT FQTA QGND IFQA VETA IHRQ KAQG KAGQ GHDV LRAD SHEG EVAE GKLP |
| Gene ID: | 1796 |
| Uniprot: | Q99704 |
| Cellular Location: | Cytoplasm, Nucleus, perinuclear region |
| Calculated MW: | 19kDa/37kDa/52kDa |
| Observed MW: | 52kDa |
| Synonyms: | DOK1, P62DOK, pp62 |
| Background: | The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | DOK1: a docking protein that interacts with receptor tyrosine kinases. It is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 2p13 Cellular Component: perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus Molecular Function:protein binding; receptor signaling protein activity; insulin receptor binding Biological Process: cell surface receptor linked signal transduction; Ras protein signal transduction; insulin receptor signaling pathway; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway |
| NCBI Summary: | The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010] |
| UniProt Code: | Q99704 |
| NCBI GenInfo Identifier: | 17366642 |
| NCBI Gene ID: | 1796 |
| NCBI Accession: | Q99704.1 |
| UniProt Secondary Accession: | Q99704,O43204, Q53TY2, Q9UHG6, |
| UniProt Related Accession: | Q99704 |
| Molecular Weight: | 481 |
| NCBI Full Name: | Docking protein 1 |
| NCBI Synonym Full Names: | docking protein 1, 62kDa (downstream of tyrosine kinase 1) |
| NCBI Official Symbol: | DOK1 |
| NCBI Official Synonym Symbols: | P62DOK |
| NCBI Protein Information: | docking protein 1; pp62; p62(dok); Downstream of tyrosine kinase 1; docking protein 1 (downstream of tyrosine kinase 1) |
| UniProt Protein Name: | Docking protein 1 |
| UniProt Synonym Protein Names: | Downstream of tyrosine kinase 1; p62(dok); pp62 |
| Protein Family: | Docking protein |
| UniProt Gene Name: | DOK1 |
| UniProt Entry Name: | DOK1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DOK1 antibody (CAB5687) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using DOK1 Antibody (CAB5687) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using DOK1 Antibody (CAB5687) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using DOK1 Antibody (CAB5687) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using DOK1 antibody (CAB5687). Blue: DAPI for nuclear staining. |
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