Anti-DNTT 항체 (CAB6254)

Antibody Name:	Anti-DNTT Antibody
Antibody SKU:	CAB6254
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 260-509 of human DNTT (NP_004079.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse thymus, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 260-509 of human DNTT (NP_004079.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LKTS EKWF RMGF RTLS KVRS DKSL KFTR MQKA GFLY YEDL VSCV TRAE AEAV SVLV KEAV WAFL PDAF VTMT GGFR RGKK MGHD VDFL ITSP GSTE DEEQ LLQK VMNL WEKK GLLL YYDL VEST FEKL RLPS RKVD ALDH FQKC FLIF KLPR QRVD SDQS SWQE GKTW KAIR VDLV LCPY ERRA FALL GWTG SRQF ERDL RRYA THER KMIL DNHA LYDK TKRI FLKA ESEE EIFA HLGL DYIE PWER NA
Gene ID:	1791
Uniprot:	P04053
Cellular Location:	Nucleus
Calculated MW:	58kDa
Observed MW:	70kDa

Synonyms:

DNTT, TDT

Background:

This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

UniProt Protein Function:	DNTT: Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. Belongs to the DNA polymerase type-X family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.7.7.31; Transferase Chromosomal Location of Human Ortholog: 10q23-q24 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:DNA nucleotidylexotransferase activity; protein binding Biological Process: DNA metabolic process
NCBI Summary:	This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	P04053
NCBI GenInfo Identifier:	311033533
NCBI Gene ID:	1791
NCBI Accession:	P04053.3
UniProt Secondary Accession:	P04053,Q53FH1, Q5W103, Q96E50,
UniProt Related Accession:	P04053
Molecular Weight:	58,408 Da
NCBI Full Name:	DNA nucleotidylexotransferase
NCBI Synonym Full Names:	DNA nucleotidylexotransferase
NCBI Official Symbol:	DNTT
NCBI Official Synonym Symbols:	TDT
NCBI Protein Information:	DNA nucleotidylexotransferase
UniProt Protein Name:	DNA nucleotidylexotransferase
UniProt Synonym Protein Names:	Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase
Protein Family:	DNA nucleotidylexotransferase
UniProt Gene Name:	DNTT
UniProt Entry Name:	TDT_HUMAN

Western blot analysis of extracts of various cell lines, using DNTT antibody (CAB6254) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies	ELISA Kits
DNTT Antibody (PACO02593)	Human TdT / DNA nucleotidylexotransferase ELISA Kit
	Human DNA nucleotidylexotransferase (DNTT) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)