Anti-DNMT3A Antibody (CAB2065)
- SKU:
- CAB2065
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-DNMT3A Antibody |
| Antibody SKU: | CAB2065 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human DNMT3A (NP_715640.2). |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:20 - 1:100 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HeLa, HL-60, MCF7, HepG2, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human DNMT3A (NP_715640.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SVTQ KHIQ EWGP FDLV IGGS PCND LSIV NPAR KGLY EGTG RLFF EFYR LLHD ARPK EGDD RPFF WLFE NVVA MGVS DKRD ISRF LESN PVMI DAKE VSAA HRAR YFWG NLPG MNRP LAST VNDK LELQ ECLE HGRI AKFS KVRT ITTR SNSI KQGK DQHF PVFM NEKE DILW CTEM ERVF GFPV HYTD VSNM SRLA RQRL L |
| Gene ID: | 1788 |
| Uniprot: | Q9Y6K1 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 16kDa/81kDa/101kDa |
| Observed MW: | 110kDa |
| Synonyms: | DNMT3A, DNMT3A2, M.HsaIIIA, TBRS, DNMT3A |
| Background: | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. |
| UniProt Protein Function: | DNMT3A: a DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Prefers CpG methylation to CpA, CpT, and CpC methylation. Methylates CpG sites at a rate much slower than DNMT1, but greater than DNMT3b. Methylation is coordinated with methylation of histones. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1 and DNMT1. Can co-localize with heterochromatin protein (HP1 ) and methyl-CpG binding protein (MeCBP). Associates with HDAC1 through its ADD-type zinc-finger. Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. |
| UniProt Protein Details: | Protein type:EC 2.1.1.37; Methyltransferase, DNA; Amino Acid Metabolism - cysteine and methionine; Methyltransferase Chromosomal Location of Human Ortholog: 2p23 Cellular Component: cytoplasm; euchromatin; nuclear matrix; nucleoplasm; nucleus Molecular Function:DNA (cytosine-5-)-methyltransferase activity; DNA binding; DNA-methyltransferase activity; identical protein binding; protein binding Biological Process: DNA methylation; genetic imprinting; negative regulation of gene expression, epigenetic Disease: Tatton-brown-rahman Syndrome |
| NCBI Summary: | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016] |
| UniProt Code: | Q9Y6K1 |
| NCBI GenInfo Identifier: | 166215081 |
| NCBI Gene ID: | 1788 |
| NCBI Accession: | Q9Y6K1.4 |
| UniProt Secondary Accession: | Q9Y6K1,Q86TE8, Q86XF5, Q8IZV0, Q8WXU9, E9PEB8, |
| UniProt Related Accession: | Q9Y6K1 |
| Molecular Weight: | 16,866 Da |
| NCBI Full Name: | DNA (cytosine-5)-methyltransferase 3A |
| NCBI Synonym Full Names: | DNA methyltransferase 3 alpha |
| NCBI Official Symbol: | DNMT3A |
| NCBI Official Synonym Symbols: | TBRS; DNMT3A2; M.HsaIIIA |
| NCBI Protein Information: | DNA (cytosine-5)-methyltransferase 3A |
| UniProt Protein Name: | DNA (cytosine-5)-methyltransferase 3A |
| UniProt Synonym Protein Names: | DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; M.HsaIIIA |
| Protein Family: | DNA |
| UniProt Gene Name: | DNMT3A |
| UniProt Entry Name: | DNM3A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DNMT3A antibody (CAB2065) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of U2OS cells using DNMT3A antibody (CAB2065). |
 | Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug DNMT3A antibody (CAB2065). Western blot was performed from the immunoprecipitate using DNMT3A antibody (CAB2065) at a dilition of 1:1000. |
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