Anti-DNAJC10 Antibody (CAB12143)

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SKU:
CAB12143
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-DNAJC10 Antibody
Antibody SKU:CAB12143
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse,
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 614-793 of human DNAJC10 (NP_061854.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse,
Positive Samples:U-87MG, HT-29, SKOV3, Raji, SGC-7901
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 614-793 of human DNAJC10 (NP_061854.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IDCQ QYHS FCAQ ENVQ RYPE IRFF PPKS NKAY HYHS YNGW NRDA YSLR IWGL GFLP QVST DLTP QTFS EKVL QGKN HWVI DFYA PWCG PCQN FAPE FELL ARMI KGKV KAGK VDCQ AYAQ TCQK AGIR AYPT VKFY FYER AKRN FQEE QINT RDAK AIAA LISE KLET LRNQ GKRN KDEL
Gene ID:54431
Uniprot:Q8IXB1
Cellular Location:Endoplasmic reticulum lumen
Calculated MW:37kDa/86kDa/91kDa
Observed MW:91kDa
Synonyms:DNAJC10, ERdj5, JPDI, MTHr, PDIA19
Background:This gene encodes an endoplasmic reticulum co-chaperone which is part of the endoplasmic reticulum-associated degradation complex involved in recognizing and degrading misfolded proteins. The encoded protein reduces incorrect disulfide bonds in misfolded glycoproteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
UniProt Protein Function:DNAJC10: This endoplasmic reticulum co-chaperone may play a role in protein folding and translocation across the endoplasmic reticulum membrane. May act as a co-chaperone for HSPA5. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Chaperone; Endoplasmic reticulum; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q32.1

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; membrane

Molecular Function:ATPase activator activity; ATPase binding; chaperone binding; disulfide oxidoreductase activity; Hsp70 protein binding; misfolded protein binding; oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor; protein binding; protein disulfide oxidoreductase activity

Biological Process: ER-associated protein catabolic process; negative regulation of protein amino acid phosphorylation; positive regulation of ATPase activity

NCBI Summary:This gene encodes an endoplasmic reticulum co-chaperone which is part of the endoplasmic reticulum-associated degradation complex involved in recognizing and degrading misfolded proteins. The encoded protein reduces incorrect disulfide bonds in misfolded glycoproteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012]
UniProt Code:Q8IXB1
NCBI GenInfo Identifier:142981524
NCBI Gene ID:54431
NCBI Accession:Q8IXB1.2
UniProt Secondary Accession:Q8IXB1,Q17RJ6, Q3B7W8, Q4ZG06, Q53QT7, Q6UWZ6, Q86T61 Q8NC82, Q8TD87, Q96K38, Q96K44, Q96K54,
UniProt Related Accession:Q8IXB1
Molecular Weight:91kDa
NCBI Full Name:DnaJ homolog subfamily C member 10
NCBI Synonym Full Names:DnaJ heat shock protein family (Hsp40) member C10
NCBI Official Symbol:DNAJC10  
NCBI Official Synonym Symbols:JPDI; MTHr; ERdj5; PDIA19  
NCBI Protein Information:dnaJ homolog subfamily C member 10
UniProt Protein Name:DnaJ homolog subfamily C member 10
UniProt Synonym Protein Names:Endoplasmic reticulum DNA J domain-containing protein 5; ER-resident protein ERdj5; ERdj5; Macrothioredoxin; MTHr
Protein Family:DnaJ homolog subfamily
UniProt Gene Name:DNAJC10  
Anti-DNAJC10 Antibody (CAB12143)Western blot analysis of extracts of various cell lines, using DNAJC10 antibody (CAB12143) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-DNAJC10 Antibody (CAB12143)Immunofluorescence analysis of L929 cells using DNAJC10 Rabbit pAb (CAB12143) at dilution of 1:100. Blue: DAPI for nuclear staining.

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