Anti-DNAJB12 Antibody (CAB14625)
- SKU:
- CAB14625
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| Antibody Name: | Anti-DNAJB12 Antibody |
| Antibody SKU: | CAB14625 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-409 of human DNAJB12 (NP_060096.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HT-29, BxPC-3, Mouse kidney, Mouse liver, Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-409 of human DNAJB12 (NP_060096.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NVHV YSNG RMRY TYQQ RQDR RDNQ GDGG LGVF VQLM PILI LILV SALS QLMV SSPP YSLS PRPS VGHI HRRV TDHL GVVY YVGD TFSE EYTG SSLK TVER NVED DYIA NLRN NCWK EKQQ KEGL LYRA RYFG DTDM YHRA QKMG TPSC SRLS EVQA SLHG |
| Gene ID: | 54788 |
| Uniprot: | Q9NXW2 |
| Cellular Location: | Membrane, Single-pass membrane protein |
| Calculated MW: | 41kDa/42kDa |
| Observed MW: | 41kDa |
| Synonyms: | DNAJB12, DJ10 |
| Background: |
| UniProt Protein Function: | DNAJB12: 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Chaperone; Membrane protein, integral Chromosomal Location of Human Ortholog: 10q22.1 Cellular Component: membrane; integral to membrane |
| NCBI Summary: | DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9NXW2 |
| NCBI GenInfo Identifier: | 294862531 |
| NCBI Gene ID: | 54788 |
| NCBI Accession: | Q9NXW2.4 |
| UniProt Secondary Accession: | Q9NXW2,Q9H6H0, B7Z7I3, |
| UniProt Related Accession: | Q9NXW2 |
| Molecular Weight: | 375 |
| NCBI Full Name: | DnaJ homolog subfamily B member 12 |
| NCBI Synonym Full Names: | DnaJ (Hsp40) homolog, subfamily B, member 12 |
| NCBI Official Symbol: | DNAJB12 |
| NCBI Official Synonym Symbols: | DJ10 |
| NCBI Protein Information: | dnaJ homolog subfamily B member 12 |
| UniProt Protein Name: | DnaJ homolog subfamily B member 12 |
| Protein Family: | DnaJ homolog subfamily |
| UniProt Gene Name: | DNAJB12 |
| UniProt Entry Name: | DJB12_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DNAJB12 antibody (CAB14625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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