Anti-DNAJA1 Antibody (CAB19865)[KO Validated]

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SKU:
CAB19865
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-DNAJA1 Antibody [KO Validated]
Antibody SKU:CAB19865
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human DNAJA1.
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant protein of human DNAJA1.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:3301
Uniprot:P31689
Cellular Location:Cytoplasm, Lipid-anchor, Membrane, Microsome, Mitochondrion, Nucleus, perinuclear region
Calculated MW:37kDa/44kDa
Observed MW:47KDa
Anti-DNAJA1 Antibody [KO Validated] (CAB19865)Western blot analysis of extracts from normal (control) and DNAJA1 knockout (KO) 293T cells, using DNAJA1 antibody (CAB19865) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:DNAJA1, DJ-2, DjA1, HDJ2, HSDJ, HSJ-2, HSJ2, HSPF4, NEDD7, hDJ-2
Background:This gene encodes a member of the DnaJ family of proteins, which act as heat shock protein 70 cochaperones. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. In humans, this gene has been implicated in positive regulation of virus replication through co-option by the influenza A virus. Several pseudogenes of this gene are found on other chromosomes.
UniProt Protein Function:DNAJA1: Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria.
UniProt Protein Details:

Protein type:Chaperone; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: membrane; mitochondrion; perinuclear region of cytoplasm; cytosol; nucleus; ubiquitin ligase complex

Molecular Function:protein binding; G-protein-coupled receptor binding; low-density lipoprotein receptor binding; chaperone binding; metal ion binding; ubiquitin protein ligase binding; unfolded protein binding; Hsp70 protein binding; ATP binding

Biological Process: negative regulation of JNK activity; protein folding; positive regulation of apoptosis; DNA damage response, detection of DNA damage; response to unfolded protein; regulation of protein transport; sperm motility; response to heat; androgen receptor signaling pathway; spermatogenesis; protein refolding; negative regulation of protein ubiquitination; negative regulation of apoptosis

UniProt Code:P31689
NCBI GenInfo Identifier:1706474
NCBI Gene ID:3301
NCBI Accession:P31689.2
UniProt Secondary Accession:P31689,Q5T7Q0, Q86TL9,
UniProt Related Accession:P31689
Molecular Weight:397
NCBI Full Name:DnaJ homolog subfamily A member 1
NCBI Synonym Full Names:DnaJ (Hsp40) homolog, subfamily A, member 1
NCBI Official Symbol:DNAJA1  
NCBI Official Synonym Symbols:DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7; hDJ-2  
NCBI Protein Information:dnaJ homolog subfamily A member 1; HSJ-2; human DnaJ protein 2; heat shock protein J2; dnaJ protein homolog 2; heat shock 40 kDa protein 4; heat shock protein, DNAJ-like 2; neural precursor cell expressed, developmentally down-regulated 7
UniProt Protein Name:DnaJ homolog subfamily A member 1
UniProt Synonym Protein Names:DnaJ protein homolog 2; HSDJ; Heat shock 40 kDa protein 4; Heat shock protein J2; HSJ-2; Human DnaJ protein 2; hDj-2
UniProt Gene Name:DNAJA1  
UniProt Entry Name:DNJA1_HUMAN

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