Anti-DNAI1 Antibody (CAB12130)
- SKU:
- CAB12130
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-DNAI1 Antibody |
| Antibody SKU: | CAB12130 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-699 of human DNAI1 (NP_036276.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, SKOV3, U-87MG, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-699 of human DNAI1 (NP_036276.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VGYG SYDF MKQS RGML LLYS LKNP SFPE YMFS SNSG VMCL DIHV DHPY LVAV GHYD GNVA IYNL KKPH SQPS FCSS AKSG KHSD PVWQ VKWQ KDDM DQNL NFFS VSSD GRIV SWTL VKRK LVHI DVIK LKVE GSTT EVPE GLQL HPVG CGTA FDFH KEID YMFL VGTE EGKI YKCS KSYS SQFL DTYD AHNM SVDT VSWN PYHT KVFM SCSS DWTV KIWD HTIK TPMF IYDL NSAV GDVA WAPY SSTV FAAV TTDG KAHI FDLA INKY EAIC NQPV AAKK NRLT HVQF NLIH PIII VGDD RGHI ISLK LSPN LRKM PKEK KGQE VQKG PAVE IAKL DKLL NLVR EVKI KT |
| Gene ID: | 27019 |
| Uniprot: | Q9UI46 |
| Cellular Location: | Cytoplasm, cilium axoneme, cytoskeleton |
| Calculated MW: | 19kDa/79kDa |
| Observed MW: | 79kDa |
| Synonyms: | DNAI1, CILD1, DIC1, ICS1, PCD |
| Background: | This gene encodes a member of the dynein intermediate chain family. The encoded protein is part of the dynein complex in respiratory cilia. The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia and Kartagener syndrome. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | DNAI1: Part of the dynein complex of respiratory cilia. Defects in DNAI1 are the cause of primary ciliary dyskinesia type 1 (CILD1). CILD1 is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome. Defects in DNAI1 are the cause of Kartagener syndrome (KTGS). KTGS is an autosomal recessive disorder characterized by the association of primary ciliary dyskinesia with situs inversus. Clinical features include recurrent respiratory infections, bronchiectasis, infertility, and lateral transposition of the viscera of the thorax and abdomen. The situs inversus is most often total, although it can be partial in some cases (isolated dextrocardia or isolated transposition of abdominal viscera). Belongs to the dynein intermediate chain family. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 9p13.3 Cellular Component: cilium; cytoskeleton Molecular Function:protein binding Biological Process: determination of left/right symmetry; sperm motility Disease: Ciliary Dyskinesia, Primary, 1 |
| NCBI Summary: | This gene encodes a member of the dynein intermediate chain family. The encoded protein is part of the dynein complex in respiratory cilia. The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia and Kartagener syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q9UI46 |
| NCBI GenInfo Identifier: | 12643888 |
| NCBI Gene ID: | 27019 |
| NCBI Accession: | Q9UI46.1 |
| UniProt Secondary Accession: | Q9UI46,Q5T8G7, Q8NHQ7, Q9UEZ8, B7Z7U1, |
| UniProt Related Accession: | Q9UI46 |
| Molecular Weight: | 79kDa |
| NCBI Full Name: | Dynein intermediate chain 1, axonemal |
| NCBI Synonym Full Names: | dynein axonemal intermediate chain 1 |
| NCBI Official Symbol: | DNAI1 |
| NCBI Official Synonym Symbols: | PCD; DIC1; ICS1; CILD1 |
| NCBI Protein Information: | dynein intermediate chain 1, axonemal |
| UniProt Protein Name: | Dynein intermediate chain 1, axonemal |
| UniProt Synonym Protein Names: | Axonemal dynein intermediate chain 1 |
| Protein Family: | Dynein |
| UniProt Gene Name: | DNAI1 |
 | Western blot analysis of extracts of various cell lines, using DNAI1 antibody (CAB12130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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