Anti-DKK1 Antibody (CAB2562)
- SKU:
- CAB2562
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-DKK1 Antibody |
| Antibody SKU: | CAB2562 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 71-266 of human DKK1 (NP_036374.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, K-562, Mouse brain, Rat brain, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 71-266 of human DKK1 (NP_036374.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GNKY QTID NYQP YPCA EDEE CGTD EYCA SPTR GGDA GVQI CLAC RKRR KRCM RHAM CCPG NYCK NGIC VSSD QNHF RGEI EETI TESF GNDH STLD GYSR RTTL SSKM YHTK GQEG SVCL RSSD CASG LCCA RHFW SKIC KPVL KEGQ VCTK HRRK GSHG LEIF QRCY CGEG LSCR IQKD HHQA SNSS RLHT CQRH |
| Gene ID: | 22943 |
| Uniprot: | O94907 |
| Cellular Location: | Secreted |
| Calculated MW: | 28kDa |
| Observed MW: | 28-45kDa |
| Synonyms: | DKK1, DKK-1, SK |
| Background: | This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. |
| UniProt Protein Function: | DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family. |
| UniProt Protein Details: | Protein type:Inhibitor; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 10q11.2 Cellular Component: early endosome membrane; extracellular region; extracellular space; plasma membrane Molecular Function:growth factor activity; low-density lipoprotein receptor binding; protein binding; receptor antagonist activity; signal transducer activity Biological Process: negative regulation of mesodermal cell fate specification; negative regulation of peptidyl-serine phosphorylation; negative regulation of protein binding; negative regulation of synaptogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of Wnt receptor signaling pathway; regulation of endodermal cell fate specification; regulation of receptor internalization |
| NCBI Summary: | This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O94907 |
| NCBI GenInfo Identifier: | 13124053 |
| NCBI Gene ID: | 22943 |
| NCBI Accession: | O94907.1 |
| UniProt Secondary Accession: | O94907,B2RC19, |
| UniProt Related Accession: | O94907 |
| Molecular Weight: | 28,672 Da |
| NCBI Full Name: | Dickkopf-related protein 1 |
| NCBI Synonym Full Names: | dickkopf WNT signaling pathway inhibitor 1 |
| NCBI Official Symbol: | DKK1 |
| NCBI Official Synonym Symbols: | SK; DKK-1 |
| NCBI Protein Information: | dickkopf-related protein 1 |
| UniProt Protein Name: | Dickkopf-related protein 1 |
| UniProt Synonym Protein Names: | SK |
| Protein Family: | Dickkopf-related protein |
| UniProt Gene Name: | DKK1 |
| UniProt Entry Name: | DKK1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DKK1 antibody (CAB2562) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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