Anti-DHRS9 Antibody (CAB6324)
- SKU:
- CAB6324
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-DHRS9 Antibody |
| Antibody SKU: | CAB6324 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-319 of human DHRS9 (NP_001135743.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | THP-1, Mouse trachea, HT-29, Mouse pancreas, Rat trachea |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-319 of human DHRS9 (NP_001135743.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RKGK LKIE DITD KYIF ITGC DSGF GNLA ARTF DKKG FHVI AACL TESG STAL KAET SERL RTVL LDVT DPEN VKRT AQWV KNQV GEKG LWGL INNA GVPG VLAP TDWL TLED YREP IEVN LFGL ISVT LNML PLVK KAQG RVIN VSSV GGRL AIVG GGYT PSKY AVEG FNDS LRRD MKAF GVHV SCIE PGLF KTNL ADPV KVIE KKLA IWEQ LSPD IKQQ YGEG YIEK SLDK LKGN KSYV NMDL SPVV ECMD HALT SLFP KTHY AAGK DAKI FWIP LSHM PAAL QDFL LLKQ KAEL ANPK AV |
| Gene ID: | 10170 |
| Uniprot: | Q9BPW9 |
| Cellular Location: | Endoplasmic reticulum membrane, Microsome membrane |
| Calculated MW: | 18kDa/30kDa/35kDa/41kDa |
| Observed MW: | 35kDa |
| Synonyms: | DHRS9, 3-alpha-HSD, 3ALPHA-HSD, RDH-E2, RDH-TBE, RDH15, RDHL, RDHTBE, RETSDR8, SDR9C4 |
| Background: | This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. This protein demonstrates oxidoreductase activity toward hydroxysteroids and is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | DHRS9: 3-alpha-hydroxysteroid dehydrogenase that converts 3- alpha-tetrahydroprogesterone (allopregnanolone) to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone. May play a role in the biosynthesis of retinoic acid from retinaldehyde, but seems to have low activity with retinoids. Can utilize both NADH and NADPH. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Cofactor and Vitamin Metabolism - retinol; Endoplasmic reticulum; Secreted; EC 1.1.-.-; Oxidoreductase Chromosomal Location of Human Ortholog: 2q31.1 Cellular Component: endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane Molecular Function:alcohol dehydrogenase activity; racemase and epimerase activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity; retinol dehydrogenase activity Biological Process: epithelial cell differentiation; retinol metabolic process; androgen metabolic process; 9-cis-retinoic acid biosynthetic process; progesterone metabolic process |
| NCBI Summary: | This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. This protein demonstrates oxidoreductase activity toward hydroxysteroids and is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| UniProt Code: | Q9BPW9 |
| NCBI GenInfo Identifier: | 74752227 |
| NCBI Gene ID: | 10170 |
| NCBI Accession: | Q9BPW9.1 |
| UniProt Secondary Accession: | Q9BPW9,Q5RKX1, Q9NRA9, Q9NRB0, B7Z416, D3DPC1, |
| UniProt Related Accession: | Q9BPW9 |
| Molecular Weight: | 319 |
| NCBI Full Name: | Dehydrogenase/reductase SDR family member 9 |
| NCBI Synonym Full Names: | dehydrogenase/reductase (SDR family) member 9 |
| NCBI Official Symbol: | DHRS9 |
| NCBI Official Synonym Symbols: | RDHL; RDH15; RDH-E2; RDHTBE; SDR9C4; RDH-TBE; RETSDR8; 3ALPHA-HSD; 3-alpha-HSD |
| NCBI Protein Information: | dehydrogenase/reductase SDR family member 9; retinol dehydrogenase homolog; 3-alpha hydroxysteroid dehydrogenase; short-chain dehydrogenase/reductase retSDR8; NADP-dependent retinol dehydrogenase/reductase; short chain dehydrogenase/reductase family 9C, member 4; tracheobronchial epithelial cell-specific retinol dehydrogenase |
| UniProt Protein Name: | Dehydrogenase/reductase SDR family member 9 |
| UniProt Synonym Protein Names: | 3-alpha hydroxysteroid dehydrogenase; 3-alpha-HSD; NADP-dependent retinol dehydrogenase/reductase; RDH-E2; RDHL; Short-chain dehydrogenase/reductase retSDR8; Tracheobronchial epithelial cell-specific retinol dehydrogenase; RDH-TBE |
| Protein Family: | Dehydrogenase/reductase SDR family |
| UniProt Gene Name: | DHRS9 |
| UniProt Entry Name: | DHRS9_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DHRS9 antibody (CAB6324) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 390s. |
 | Western blot analysis of extracts of various cell lines, using DHRS9 antibody (CAB6324) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat brain using DHRS9 Antibody (CAB6324) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using DHRS9 Antibody (CAB6324) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using DHRS9 antibody (CAB6324). Blue: DAPI for nuclear staining. |
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