Anti-DHPS Antibody (CAB6367)
- SKU:
- CAB6367
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-DHPS Antibody |
| Antibody SKU: | CAB6367 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human DHPS (NP_001921.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, BT-474, 293T, Jurkat, SGC-7901, Mouse testis, Mouse brain, Mouse skeletal muscle, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human DHPS (NP_001921.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEGS LERE APAG ALAA VLKH SSTL PPES TQVR GYDF NRGV NYRA LLEA FGTT GFQA TNFG RAVQ QVNA MIEK KLEP LSQD EDQH ADLT QSRR PLTS CTIF LGYT SNLI SSGI RETI RYLV QHNM VDVL VTTA GGVE EDLI KCLA PTYL GEFS LRGK ELRE NGIN RIGN LLVP NENY CKFE DWLM PILD QMVM EQNT EGVK WTPS KMIA RLGK EINN PESV YYWA QKNH IPVF SPAL TDGS LGDM IFFH SYKN PGLV LDIV EDLR LINT QAIF AKCT GMII LGGG VVKH HIAN ANLM RNGA DYAV YINT AQEF DGSD SGAR PDEA VSWG KIRV DAQP VKVY ADAS LVFP LLVA ETFA QKMD AFMH EKNE D |
| Gene ID: | 1725 |
| Uniprot: | P49366 |
| Cellular Location: | |
| Calculated MW: | 35kDa/36kDa/40kDa |
| Observed MW: | 41kDa |
| Synonyms: | DHPS, DHS, DS, MIG13 |
| Background: | This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | DHPS: Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Belongs to the deoxyhypusine synthase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; EC 2.5.1.46; Transferase Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: cytosol Molecular Function:deoxyhypusine synthase activity; protein binding Biological Process: hypusine biosynthetic process from peptidyl-lysine; positive regulation of cell proliferation; translation |
| NCBI Summary: | This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2011] |
| UniProt Code: | P49366 |
| NCBI GenInfo Identifier: | 1352267 |
| NCBI Gene ID: | 1725 |
| NCBI Accession: | P49366.1 |
| UniProt Secondary Accession: | P49366,Q13184, Q13276, Q9UDG0, A8K688, M0R1I5, |
| UniProt Related Accession: | P49366 |
| Molecular Weight: | 36,583 Da |
| NCBI Full Name: | Deoxyhypusine synthase |
| NCBI Synonym Full Names: | deoxyhypusine synthase |
| NCBI Official Symbol: | DHPS |
| NCBI Official Synonym Symbols: | DS; DHS; MIG13 |
| NCBI Protein Information: | deoxyhypusine synthase |
| UniProt Protein Name: | Deoxyhypusine synthase |
| UniProt Gene Name: | DHPS |
| UniProt Entry Name: | DHYS_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DHPS antibody (CAB6367) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5s. |
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