Anti-DHDDS 항체 (CAB7835)

Antibody Name:	Anti-DHDDS Antibody
Antibody SKU:	CAB7835
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human DHDDS (NP_995583.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Mouse testis, Rat thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human DHDDS (NP_995583.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IKAG PMPK HIAF IMDG NRRY AKKC QVER QEGH SQGF NKLA ETLR WCLN LGIL EVTV YAFS IENF KRSK SEVD GLMD LARQ KFSR LMEE KEKL QKHG VCIR VLGD LHLL PLDL QELI AQAV QATK NYNK CFLN VCFA YTSR HEIS NAVR EMAW GVEQ GLLD PSDI SESL LDKC LYTN RSPH P
Gene ID:	79947
Uniprot:	Q86SQ9
Cellular Location:	Endoplasmic reticulum membrane, Peripheral membrane protein
Calculated MW:	34kDa/38kDa
Observed MW:	34kDa

Synonyms:	DHDDS, CIT, CPT, DS, HDS, RP59
Background:	The protein encoded by this gene catalyzes cis-prenyl chain elongation to produce the polyprenyl backbone of dolichol, a glycosyl carrier lipid required for the biosynthesis of several classes of glycoproteins. Mutations in this gene are associated with retinitis pigmentosa type 59. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

UniProt Protein Function:	DHDDS: Catalyzes cis-prenyl chain elongation to produce the polyprenyl backbone of dolichol, a glycosyl carrier-lipid required for the biosynthesis of several classes of glycoprotein. Defects in DHDDS are the cause of retinitis pigmentosa type 59 (RP59). RP59 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Belongs to the UPP synthase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Transferase; EC 2.5.1.87 Chromosomal Location of Human Ortholog: 1p36.11 Cellular Component: endoplasmic reticulum membrane Molecular Function:transferase activity, transferring alkyl or aryl (other than methyl) groups; protein binding Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; dolichyl diphosphate biosynthetic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification Disease: Retinitis Pigmentosa 59
NCBI Summary:	The protein encoded by this gene catalyzes cis-prenyl chain elongation to produce the polyprenyl backbone of dolichol, a glycosyl carrier lipid required for the biosynthesis of several classes of glycoproteins. Mutations in this gene are associated with retinitis pigmentosa type 59. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
UniProt Code:	Q86SQ9
NCBI GenInfo Identifier:	343790880
NCBI Gene ID:	79947
NCBI Accession:	NP_001230494.1
UniProt Related Accession:	Q86SQ9
Molecular Weight:	39kDa
NCBI Full Name:	dehydrodolichyl diphosphate synthase complex subunit DHDDS isoform 4
NCBI Synonym Full Names:	dehydrodolichyl diphosphate synthase subunit
NCBI Official Symbol:	DHDDS
NCBI Official Synonym Symbols:	DS; CIT; CPT; HDS; RP59; hCIT; DEDSM
NCBI Protein Information:	dehydrodolichyl diphosphate synthase complex subunit DHDDS
UniProt Protein Name:	Dehydrodolichyl diphosphate synthase
UniProt Synonym Protein Names:	Cis-isoprenyltransferase; CIT; Cis-IPTase; Epididymis tissue protein Li 189m
Protein Family:	Dehydrodolichyl diphosphate synthase complex
UniProt Gene Name:	DHDDS
UniProt Entry Name:	DHDDS_HUMAN

Western blot analysis of extracts of various cell lines, using DHDDS antibody (CAB7835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.

Antibodies

DHDDS Antibody (PACO08819)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)