Anti-DET1 homolog Antibody (CAB9974)
- SKU:
- CAB9974
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-DET1 homolog Antibody |
| Antibody SKU: | CAB9974 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 15-240 of human DET1 (NP_060466.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | BT-474, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 15-240 of human DET1 (NP_060466.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HVST IKPR RIQN QNVI HRLE RRRI SSGK AGTH WHQV RVFH QNVF PNFT VVNV EKPP CFLR KFSP DGRY FIAF SSDQ TSLE IYEY QGCQ AAED LLQG YEGE ILSN GNDQ RSVN IRGR LFER FFVL LHIT NVAA NGEH LNRE CSLF TDDC RCVI VGSA AYLP DEPH PPFF EVYR NSES VTPN PRSP LEDY SLHI IDLH TGRL CDTR TFKC DKVV LSHN QGLY LYKN IL |
| Gene ID: | 55070 |
| Uniprot: | Q7L5Y6 |
| Cellular Location: | Nucleus |
| Calculated MW: | 63kDa/65kDa |
| Observed MW: | 55kDa |
| Synonyms: | DET1 |
| Background: |
| UniProt Protein Function: | DET1: Component of the E3 ubiquitin ligase DCX DET1-COP1 complex, which is required for ubiquitination and subsequent degradation of target proteins. The complex is involved in JUN ubiquitination and degradation. Belongs to the DET1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase Chromosomal Location of Human Ortholog: 15q26.1 |
| UniProt Code: | Q7L5Y6 |
| NCBI GenInfo Identifier: | 55976471 |
| NCBI Gene ID: | 55070 |
| NCBI Accession: | Q7L5Y6.2 |
| UniProt Secondary Accession: | Q7L5Y6,Q2VPC0, Q9NWD5, B3KNN6, |
| UniProt Related Accession: | Q7L5Y6 |
| Molecular Weight: | 64kDa |
| NCBI Full Name: | DET1 homolog |
| NCBI Synonym Full Names: | de-etiolated homolog 1 (Arabidopsis) |
| NCBI Official Symbol: | DET1 |
| NCBI Protein Information: | DET1 homolog |
| UniProt Protein Name: | DET1 homolog |
| UniProt Synonym Protein Names: | De-etiolated-1 homolog |
| Protein Family: | DET1 |
| UniProt Gene Name: | DET1 |
 | Western blot analysis of extracts of various cell lines, using DET1 antibody (CAB9974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s. |
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