Anti-DDX47 Antibody (CAB10359)
- SKU:
- CAB10359
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-DDX47 Antibody |
| Antibody SKU: | CAB10359 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human DDX47 (NP_057439.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, SKOV3, LO2, HeLa, Mouse testis, Mouse lung, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human DDX47 (NP_057439.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TEVD KILK VIPR DRKT FLFS ATMT KKVQ KLQR AALK NPVK CAVS SKYQ TVEK LQQY YIFI PSKF KDTY LVYI LNEL AGNS FMIF CSTC NNTQ RTAL LLRN LGFT AIPL HGQM SQSK RLGS LNKF KAKA RSIL LATD VASR GLDI PHVD VVVN FDIP THSK DYIH RVGR TARA GRSG KAIT FVTQ YDVE LFQR IEHL IGKK LPGF PTQD DEVM MLTE RVAE AQRF ARME LREH GEKK KRSR EDAG DNDD TEGA IGVR NKVA GGKM KKRK GR |
| Gene ID: | 51202 |
| Uniprot: | Q9H0S4 |
| Cellular Location: | Nucleus, nucleolus |
| Calculated MW: | 45kDa/50kDa |
| Observed MW: | 50kDa |
| Synonyms: | DDX47, E4-DBP, HQ0256, MSTP162, RRP3 |
| Background: | This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| UniProt Protein Function: | DDX47: a protein of the DEAD box helicase family. Unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. |
| UniProt Protein Details: | Protein type:EC 3.6.4.13; Helicase; Nucleolus; RNA-binding Chromosomal Location of Human Ortholog: 12p13.1 Cellular Component: membrane; nucleolus; nucleoplasm Molecular Function:ATP-dependent RNA helicase activity; protein binding; RNA binding Biological Process: induction of apoptosis via death domain receptors; RNA secondary structure unwinding; RNA splicing; rRNA processing |
| NCBI Summary: | This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9H0S4 |
| NCBI GenInfo Identifier: | 52782792 |
| NCBI Gene ID: | 51202 |
| NCBI Accession: | Q9H0S4.1 |
| UniProt Secondary Accession: | Q9H0S4,Q96GM0, Q96NV8, Q9UI98, B3KXP4, G5E955, |
| UniProt Related Accession: | Q9H0S4 |
| Molecular Weight: | 51kDa |
| NCBI Full Name: | Probable ATP-dependent RNA helicase DDX47 |
| NCBI Synonym Full Names: | DEAD-box helicase 47 |
| NCBI Official Symbol: | DDX47 |
| NCBI Official Synonym Symbols: | RRP3; E4-DBP; HQ0256; MSTP162 |
| NCBI Protein Information: | probable ATP-dependent RNA helicase DDX47 |
| UniProt Protein Name: | Probable ATP-dependent RNA helicase DDX47 |
| UniProt Synonym Protein Names: | DEAD box protein 47 |
| Protein Family: | Probable ATP-dependent RNA helicase |
| UniProt Gene Name: | DDX47 |
 | Western blot analysis of extracts of various cell lines, using DDX47 antibody (CAB10359) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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