Anti-DDX4 Antibody (CAB15624)
- SKU:
- CAB15624
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-DDX4 Antibody |
| Antibody SKU: | CAB15624 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DDX4 (NP_077726.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DDX4 (NP_077726.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGDE DWEA EINP HMSS YVPI FEKD RYSG ENGD NFNR TPAS SSEM DDGP SRRD HFMK SGFA SGRN FGNR DAGE CNKR DNTS TMGG FGVG KSFG NRGF SNSR FEDG DSSG FWRE SSND CEDN PTRN RGFS KRGG YRDG NNSE ASGP YRRG GRGS FRGC RGGF GLGS PNND LDPD ECMQ RTGG LFGS RRPV LSGT GNGD TSQS RSGS GSER GGYK GLNE EVIT GSGK NSWK SEAE GGES SDTQ GPKV TYIP PPPP EDED SIFA HYQT GINF DKYD TILV EVSG HDAP PAIL TFEE ANLC QTLN |
| Gene ID: | 54514 |
| Uniprot: | Q9NQI0 |
| Cellular Location: | Cytoplasm, perinuclear region |
| Calculated MW: | 63kDa/75kDa/77kDa/79kDa |
| Observed MW: | 79KDa |
| Synonyms: | DDX4, VASA |
| Background: | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | DDX4: May play a role in germ cell development. May play a role in sperm motility. Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL. Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. Belongs to the DEAD box helicase family. DDX4/VASA subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.6.4.13; Helicase Chromosomal Location of Human Ortholog: 5p15.2-p13.1 Cellular Component: cytoplasm; perinuclear region of cytoplasm Molecular Function:ATP-dependent RNA helicase activity Biological Process: DNA methylation during gametogenesis; male meiosis; RNA secondary structure unwinding; sperm motility; spermatogenesis |
| NCBI Summary: | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| UniProt Code: | Q9NQI0 |
| NCBI GenInfo Identifier: | 20138033 |
| NCBI Gene ID: | 54514 |
| NCBI Accession: | Q9NQI0.2 |
| UniProt Secondary Accession: | Q9NQI0,Q5M7Z3, Q86VX0, Q9NT92, Q9NYB1, A8K8Q2, B3KSF4 D6RDK4, E9PCD8, |
| UniProt Related Accession: | Q9NQI0 |
| Molecular Weight: | 77,056 Da |
| NCBI Full Name: | Probable ATP-dependent RNA helicase DDX4 |
| NCBI Synonym Full Names: | DEAD-box helicase 4 |
| NCBI Official Symbol: | DDX4 |
| NCBI Official Synonym Symbols: | VASA |
| NCBI Protein Information: | probable ATP-dependent RNA helicase DDX4 |
| UniProt Protein Name: | Probable ATP-dependent RNA helicase DDX4 |
| UniProt Synonym Protein Names: | DEAD box protein 4; Vasa homolog |
| Protein Family: | Probable ATP-dependent RNA helicase |
| UniProt Gene Name: | DDX4 |
| UniProt Entry Name: | DDX4_HUMAN |
 | Western blot analysis of extracts of Mouse testis, using DDX4 antibody (CAB15624) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded mouse testis using DDX4 Rabbit pAb (CAB15624) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of rat testis using DDX4 Rabbit pAb (CAB15624) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse testis using DDX4 Rabbit pAb (CAB15624) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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