Anti-DDX25 Antibody (CAB15824)
- SKU:
- CAB15824
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-DDX25 Antibody |
| Antibody SKU: | CAB15824 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 284-483 of human DDX25 (NP_037396.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, Mouse eye, Mouse testis, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 284-483 of human DDX25 (NP_037396.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DSVW HFAE RIIP DPNV IKLR KEEL TLNN IRQY YVLC EHRK DKYQ ALCN IYGS ITIG QAII FCQT RRNA KWLT VEMI QDGH QVSL LSGE LTVE QRAS IIQR FRDG KEKV LITT NVCA RGID VKQV TIVV NFDL PVKQ GEEP DYET YLHR IGRT GRFG KKGL AFNM IEVD ELPS LMKI QDHF NSSI KQLN AEDM DEIE KIDY |
| Gene ID: | 29118 |
| Uniprot: | Q9UHL0 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 42kDa/54kDa |
| Observed MW: | 55kDa |
| Synonyms: | DDX25, GRTH |
| Background: | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis. |
| UniProt Code: | Q9UHL0 |
| NCBI GenInfo Identifier: | 164419732 |
| NCBI Gene ID: | 29118 |
| NCBI Accession: | NP_037396.3 |
| UniProt Secondary Accession: | Q9UHL0,Q5XVN2, Q86W81, Q8IYP1, B2R6Z0, |
| UniProt Related Accession: | Q9UHL0 |
| Molecular Weight: | 54,692 Da |
| NCBI Full Name: | ATP-dependent RNA helicase DDX25 |
| NCBI Synonym Full Names: | DEAD (Asp-Glu-Ala-Asp) box helicase 25 |
| NCBI Official Symbol: | DDX25 |
| NCBI Official Synonym Symbols: | GRTH |
| NCBI Protein Information: | ATP-dependent RNA helicase DDX25; DEAD box protein 25; DEAD (Asp-Glu-Ala-Asp) box polypeptide 25; gonadotropin-regulated testicular RNA helicase; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25 |
| UniProt Protein Name: | ATP-dependent RNA helicase DDX25 |
| UniProt Synonym Protein Names: | DEAD box protein 25; Gonadotropin-regulated testicular RNA helicase |
| Protein Family: | ATP-dependent RNA helicase |
| UniProt Gene Name: | DDX25 |
| UniProt Entry Name: | DDX25_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DDX25 antibody (CAB15824) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s. |
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