Anti-DDO Antibody (CAB15338)
- SKU:
- CAB15338
- Product type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-DDO Antibody |
| Antibody SKU: | CAB15338 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human DDO (NP_003640.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse heart, Mouse kidney, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human DDO (NP_003640.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MRPA RHWE TRFG ARDF GGFQ DCFF RDRL MDTA RIAV VGAG VVGL STAV CISK LVPR CSVT IISD KFTP DTTS DVAA GMLI PHTY PDTP IHTQ KQWF RETF NHLF AIAN SAEA GDAG VHLV SGWQ IFQS TPTE EVPF WADV VLGF RKMT EAEL KKFP QYVF GQAF TTLK CECP AYLP WLEK RIKG SGGW TLTR RIED LWEL HPSF DIVV NCSG LGSR QLAG DSKI FPVR GQVL QVQA PWVE HFIR DGSG LTYI YPGT SHVT LGGT RQKG DWNL SPDA ENSR EILS RCCA LEPS LHGA CNIR EKVG LRPY RPGV RLQT ELLA RDGQ RLPV VHHY GHGS GGIS VHWG TALE AARL VSEC VHAL RTPI PKSN L |
| Gene ID: | 8528 |
| Uniprot: | Q99489 |
| Cellular Location: | Peroxisome |
| Calculated MW: | 30kDa/33kDa/37kDa/40kDa |
| Observed MW: | 45kDa |
| Synonyms: | DDO, DASOX, DDO-1, DDO-2 |
| Background: | The protein encoded by this gene is a peroxisomal flavoprotein that catalyzes the oxidative deamination of D-aspartate and N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | Selectively catalyzes the oxidative deamination of D-aspartate and its N-methylated derivative, N-methyl D-aspartate. |
| NCBI Summary: | The protein encoded by this gene is a peroxisomal flavoprotein that catalyzes the oxidative deamination of D-aspartate and N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99489 |
| NCBI GenInfo Identifier: | 2494037 |
| NCBI Gene ID: | 8528 |
| NCBI Accession: | Q99489.1 |
| UniProt Secondary Accession: | Q99489,Q5JXM4, Q5JXM5, Q5JXM6, Q8N552, A8KAG4, |
| UniProt Related Accession: | Q99489 |
| Molecular Weight: | 33,980 Da |
| NCBI Full Name: | D-aspartate oxidase |
| NCBI Synonym Full Names: | D-aspartate oxidase |
| NCBI Official Symbol: | DDO |
| NCBI Official Synonym Symbols: | DASOX; DDO-1; DDO-2 |
| NCBI Protein Information: | D-aspartate oxidase |
| UniProt Protein Name: | D-aspartate oxidase |
| UniProt Gene Name: | DDO |
| UniProt Entry Name: | OXDD_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DDO antibody (CAB15338) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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Human DDO / D-aspartate oxidase ELISA Kit
