Anti-DDAH1 Antibody (CAB10295)
- SKU:
- CAB10295
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-DDAH1 Antibody |
| Antibody SKU: | CAB10295 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human DDAH1 (NP_036269.1). |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW480, 22Rv1, HT-29, 293T, SGC-7901, Mouse brain, Mouse kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human DDAH1 (NP_036269.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAGL GHPA AFGR ATHA VVRA LPES LGQH ALRS AKGE EVDV ARAE RQHQ LYVG VLGS KLGL QVVE LPAD ESLP DCVF VEDV AVVC EETA LITR PGAP SRRK EVDM MKEA LEKL QLNI VEMK DENA TLDG GDVL FTGR EFFV GLSK RTNQ RGAE ILAD TFKD YAVS TVPV ADGL HLKS FCSM AGPN LIAI GSSE SAQK ALKI MQQM SDHR YDKL TVPD DIAA NCIY LNIP NKGH VLLH RTPE EYPE SAKV YEKL KDHM LIPV SMSE LEKV DGLL TCCS VLIN KKVD S |
| Gene ID: | 23576 |
| Uniprot: | O94760 |
| Cellular Location: | |
| Calculated MW: | 20kDa/31kDa |
| Observed MW: | 37kDa |
| Synonyms: | DDAH1, DDAH, DDAH-1, DDAHI, HEL-S-16 |
| Background: | This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. |
| UniProt Protein Function: | DDAH1: Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. Belongs to the DDAH family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.5.3.18; Hydrolase Chromosomal Location of Human Ortholog: 1p22.3 Cellular Component: cytosol Molecular Function:catalytic activity; dimethylargininase activity Biological Process: citrulline metabolic process; nitric oxide mediated signal transduction; positive regulation of nitric oxide biosynthetic process; regulation of nitric-oxide synthase activity; regulation of systemic arterial blood pressure |
| NCBI Summary: | This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O94760 |
| NCBI GenInfo Identifier: | 6831528 |
| NCBI Gene ID: | 23576 |
| NCBI Accession: | O94760.3 |
| UniProt Secondary Accession: | O94760,Q5HYC8, Q86XK5, |
| UniProt Related Accession: | O94760 |
| Molecular Weight: | 31kDa |
| NCBI Full Name: | N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 |
| NCBI Synonym Full Names: | dimethylarginine dimethylaminohydrolase 1 |
| NCBI Official Symbol: | DDAH1 |
| NCBI Official Synonym Symbols: | DDAH; DDAHI; DDAH-1; HEL-S-16 |
| NCBI Protein Information: | N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 |
| UniProt Protein Name: | N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 |
| UniProt Synonym Protein Names: | DDAHI; Dimethylargininase-1 |
| Protein Family: | N(G),N(G)-dimethylarginine dimethylaminohydrolase |
| UniProt Gene Name: | DDAH1 |
 | Western blot analysis of extracts of various cell lines, using DDAH1 antibody (CAB10295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of NIH-3T3 cells using DDAH1 Polyclonal Antibody (CAB10295) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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