Anti-DCTN5 Antibody (CAB15915)
- SKU:
- CAB15915
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-DCTN5 Antibody |
| Antibody SKU: | CAB15915 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DCTN5 (NP_115875.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, 293T, Mouse eye, Mouse testis, Mouse kidney, Rat lung, Rat spleen |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DCTN5 (NP_115875.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MELG ELLY NKSE YIET ASGN KVSR QSVL CGSQ NIVL NGKT IVMN DCII RGDL ANVR VGRH CVVK SRSV IRPP FKKF SKGV AFFP LHIG DHVF IEED CVVN |
| Gene ID: | 84516 |
| Uniprot: | Q9BTE1 |
| Cellular Location: | Chromosome, Cytoplasm, centromere, cytoskeleton, kinetochore |
| Calculated MW: | 9kDa/16kDa/20kDa |
| Observed MW: | 20kDa |
| Synonyms: | DCTN5 |
| Background: | This gene encodes a subunit of dynactin, a component of the cytoplasmic dynein motor machinery involved in minus-end-directed transport. The encoded protein is a component of the pointed-end subcomplex and is thought to bind membranous cargo. A pseudogene of this gene is located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | dynactin 5: a subunit of dynactin, a component of the cytoplasmic dynein motor machinery involved in minus-end-directed transport. The encoded protein is a component of the pointed-end subcomplex and is thought to bind membranous cargo. A pseudogene of this gene is located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 16p12.2 Cellular Component: centrosome; cytosol Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II |
| NCBI Summary: | This gene encodes a subunit of dynactin, a component of the cytoplasmic dynein motor machinery involved in minus-end-directed transport. The encoded protein is a component of the pointed-end subcomplex and is thought to bind membranous cargo. A pseudogene of this gene is located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] |
| UniProt Code: | Q9BTE1 |
| NCBI GenInfo Identifier: | 62900103 |
| NCBI Gene ID: | 84516 |
| NCBI Accession: | Q9BTE1.1 |
| UniProt Secondary Accession: | Q9BTE1,A8K9X8, H3BN51, H3BQA4, |
| UniProt Related Accession: | Q9BTE1 |
| Molecular Weight: | 9,740 Da |
| NCBI Full Name: | Dynactin subunit 5 |
| NCBI Synonym Full Names: | dynactin 5 (p25) |
| NCBI Official Symbol: | DCTN5 |
| NCBI Protein Information: | dynactin subunit 5; dynactin 4; dynactin subunit p25 |
| UniProt Protein Name: | Dynactin subunit 5 |
| UniProt Synonym Protein Names: | Dynactin subunit p25 |
| UniProt Gene Name: | DCTN5 |
| UniProt Entry Name: | DCTN5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DCTN5 antibody (CAB15915) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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