Anti-DAZL Antibody (CAB13970)
- SKU:
- CAB13970
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-DAZL Antibody |
| Antibody SKU: | CAB13970 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 186-295 of human DAZL (NP_001342.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse testis, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 186-295 of human DAZL (NP_001342.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VYNY QMPP QWPV GEQR SYVV PPAY SAVN YHCN EVDP GAEV VPNE CSVH EATP PSGN GPQK KSVD RSIQ TVVS CLFN PENR LRNS VVTQ DDYF KDKR VHHF RRSR AMLK SV |
| Gene ID: | 1618 |
| Uniprot: | Q92904 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 33kDa/35kDa |
| Observed MW: | 38KDa |
| Synonyms: | DAZL, DAZH, DAZL1, DAZLA, SPGYLA |
| Background: | The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | DAZL: RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts. Homodimer and heterodimer. Forms a heterodimer with DAZ. Interacts with BOLL, DAZAP1 and DAZAP2. Interacts with PUM2. Testis specific. Belongs to the RRM DAZ family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Translation Chromosomal Location of Human Ortholog: 3p24.3 Cellular Component: polysome; cytoplasm; nucleus Molecular Function:identical protein binding; protein binding; translation activator activity; mRNA 3'-UTR binding; RNA binding; nucleotide binding Biological Process: positive regulation of translational initiation; positive regulation of meiosis; female meiosis II; multicellular organismal development; spermatogenesis; oocyte maturation; germ cell development |
| NCBI Summary: | The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| UniProt Code: | Q92904 |
| NCBI GenInfo Identifier: | 27805453 |
| NCBI Gene ID: | 1618 |
| NCBI Accession: | Q92904.1 |
| UniProt Secondary Accession: | Q92904,O15396, Q5HYB4, Q92909, |
| UniProt Related Accession: | Q92904 |
| Molecular Weight: | 295 |
| NCBI Full Name: | Deleted in azoospermia-like |
| NCBI Synonym Full Names: | deleted in azoospermia-like |
| NCBI Official Symbol: | DAZL |
| NCBI Official Synonym Symbols: | DAZH; DAZL1; DAZLA; SPGYLA |
| NCBI Protein Information: | deleted in azoospermia-like; DAZ homolog; DAZ-like autosomal; SPGY-like-autosomal; deleted in azoospermia-like 1; germline specific RNA binding protein; spermatogenesis gene on the Y-like autosomal |
| UniProt Protein Name: | Deleted in azoospermia-like |
| UniProt Synonym Protein Names: | DAZ homolog; DAZ-like autosomal; Deleted in azoospermia-like 1; SPGY-like-autosomal |
| Protein Family: | Deleted in azoospermia |
| UniProt Gene Name: | DAZL |
| UniProt Entry Name: | DAZL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DAZL antibody (CAB13970) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat testis using DAZL Rabbit pAb (CAB13970) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using DAZL Rabbit pAb (CAB13970) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of rat testis using DAZL Rabbit pAb (CAB13970) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse testis using DAZL Rabbit pAb (CAB13970) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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