Anti-CYP2R1 Antibody (CAB10470)
- SKU:
- CAB10470
- Product type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-CYP2R1 Antibody |
| Antibody SKU: | CAB10470 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 252-501 of human CYP2R1 (NP_078790.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 252-501 of human CYP2R1 (NP_078790.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VVYD FLSR LIEK ASVN RKPQ LPQH FVDA YLDE MDQG KNDP SSTF SKEN LIFS VGEL IIAG TETT TNVL RWAI LFMA LYPN IQGQ VQKE IDLI MGPN GKPS WDDK CKMP YTEA VLHE VLRF CNIV PLGI FHAT SEDA VVRG YSIP KGTT VITN LYSV HFDE KYWR DPEV FHPE RFLD SSGY FAKK EALV PFSL GRRH CLGE HLAR MEMF LFFT ALLQ RFHL HFPH ELVP DLKP RLGM TLQP QPYL ICAE RR |
| Gene ID: | 120227 |
| Uniprot: | Q6VVX0 |
| Cellular Location: | Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein |
| Calculated MW: | 57kDa |
| Observed MW: | 57kDa |
| Synonyms: | CYP2R1 |
| Background: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. A mutation in this gene has been associated with selective 25-hydroxyvitamin D deficiency. |
| UniProt Protein Function: | CYP2R1: Has a D-25-hydroxylase activity on both forms of vitamin D, vitamin D(2) and D(3). Defects in CYP2R1 are the cause of rickets vitamin D- dependent type 1B (VDDR1B); also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. A disorder caused by a selective deficiency of the active form of vitamin D (1,25-dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. The patients sera have low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activityand low levels of 25-hydroxyvitamin D. Belongs to the cytochrome P450 family. |
| UniProt Protein Details: | Protein type:Oxidoreductase; EC 1.14.13.15 Chromosomal Location of Human Ortholog: 11p15.2 Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm Molecular Function:iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; steroid hydroxylase activity; oxygen binding; vitamin D3 25-hydroxylase activity Biological Process: steroid metabolic process; organic acid metabolic process; vitamin metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; vitamin D metabolic process Disease: Vitamin D Hydroxylation-deficient Rickets, Type 1b |
| NCBI Summary: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. A mutation in this gene has been associated with selective 25-hydroxyvitamin D deficiency. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q6VVX0 |
| NCBI GenInfo Identifier: | 62286619 |
| NCBI Gene ID: | 120227 |
| NCBI Accession: | Q6VVX0.1 |
| UniProt Secondary Accession: | Q6VVX0,Q2M3H3, Q5RT65, |
| UniProt Related Accession: | Q6VVX0 |
| Molecular Weight: | 57,359 Da |
| NCBI Full Name: | Vitamin D 25-hydroxylase |
| NCBI Synonym Full Names: | cytochrome P450, family 2, subfamily R, polypeptide 1 |
| NCBI Official Symbol: | CYP2R1 |
| NCBI Protein Information: | vitamin D 25-hydroxylase; cytochrome P450 2R1; cytochrome P450, family 2, R1 |
| UniProt Protein Name: | Vitamin D 25-hydroxylase |
| UniProt Synonym Protein Names: | Cytochrome P450 2R1 |
| UniProt Gene Name: | CYP2R1 |
| UniProt Entry Name: | CP2R1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CYP2R1 antibody (CAB10470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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