Anti-CYP27A1 Antibody (CAB1982)
- SKU:
- CAB1982
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-CYP27A1 Antibody |
| Antibody SKU: | CAB1982 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 200-400 of human CYP27A1 (NP_000775.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, Mouse kidney, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 200-400 of human CYP27A1 (NP_000775.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NQVS DMAQ LFYY FALE AICY ILFE KRIG CLQR SIPE DTVT FVRS IGLM FQNS LYAT FLPK WTRP VLPF WKRY LDGW NAIF SFGK KLID EKLE DMEA QLQA AGPD GIQV SGYL HFLL ASGQ LSPR EAMG SLPE LLMA GVDT TSNT LTWA LYHL SKDP EIQE ALHE EVVG VVPA GQVP QHKD FAHM PLLK AVLK ETLR LYPV V |
| Gene ID: | 1593 |
| Uniprot: | Q02318 |
| Cellular Location: | Mitochondrion membrane |
| Calculated MW: | 60kDa |
| Observed MW: | 60kDa |
| Synonyms: | CYP27A1, CP27, CTX, CYP27 |
| Background: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. |
| UniProt Protein Function: | CYP27A1: Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta- cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3- 25-hydroxylase activity. Defects in CYP27A1 are the cause of cerebrotendinous xanthomatosis (CTX). CTX is a rare sterol storage disorder characterized clinically by progressive neurologic dysfunction, premature atherosclerosis, and cataracts. Belongs to the cytochrome P450 family. |
| UniProt Protein Details: | Protein type:Oxidoreductase; Lipid Metabolism - primary bile acid biosynthesis; EC 1.14.13.15; Mitochondrial Chromosomal Location of Human Ortholog: 2q35 Cellular Component: mitochondrial matrix; mitochondrial inner membrane Molecular Function:cholestanetriol 26-monooxygenase activity; iron ion binding; cholesterol 26-hydroxylase activity; heme binding; steroid hydroxylase activity; vitamin D3 25-hydroxylase activity Biological Process: bile acid biosynthetic process; cholesterol metabolic process; bile acid metabolic process; xenobiotic metabolic process; sterol metabolic process Disease: Cerebrotendinous Xanthomatosis |
| NCBI Summary: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q02318 |
| NCBI GenInfo Identifier: | 4503211 |
| NCBI Gene ID: | 1593 |
| NCBI Accession: | NP_000775.1 |
| UniProt Related Accession: | Q02318 |
| Molecular Weight: | Predicted Molecular Mass: 39kDaAccurate Molecular Mass: 43kDa |
| NCBI Full Name: | sterol 26-hydroxylase, mitochondrial |
| NCBI Synonym Full Names: | cytochrome P450 family 27 subfamily A member 1 |
| NCBI Official Symbol: | CYP27A1 |
| NCBI Official Synonym Symbols: | CTX; CP27; CYP27 |
| NCBI Protein Information: | sterol 26-hydroxylase, mitochondrial |
| UniProt Protein Name: | Sterol 26-hydroxylase, mitochondrial |
| UniProt Synonym Protein Names: | 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase; Cytochrome P-450C27/25; Cytochrome P450 27; Sterol 27-hydroxylase; Vitamin D(3) 25-hydroxylase |
| Protein Family: | FK506-binding protein |
| UniProt Gene Name: | CYP27A1 |
| UniProt Entry Name: | CP27A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CYP27A1 antibody (CAB1982) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of HeLa cells using CYP27A1 antibody (CAB1982) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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