Anti-CYLD Antibody (CAB3821)

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SKU:
CAB3821
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
Frequently bought together:

Description

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Antibody Name:Anti-CYLD Antibody
Antibody SKU:CAB3821
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 654-953 of human CYLD (NP_001035877.1).
Application:WB IHC
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, Mouse brain, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 654-953 of human CYLD (NP_001035877.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TKIM KLRK ILEK VEAA SGFT SEEK DPEE FLNI LFHH ILRV EPLL KIRS AGQK VQDC YFYQ IFME KNEK VGVP TIQQ LLEW SFIN SNLK FAEA PSCL IIQM PRFG KDFK LFKK IFPS LELN ITDL LEDT PRQC RICG GLAM YECR ECYD DPDI SAGK IKQF CKTC NTQV HLHP KRLN HKYN PVSL PKDL PDWD WRHG CIPC QNME LFAV LCIE TSHY VAFV KYGK DDSA WLFF DSMA DRDG GQNG FNIP QVTP CPEV GEYL KMSL EDLH SLDS RRIQ GCAR RLLC DAYM CMYQ SPTM SLYK
Gene ID:1540
Uniprot:Q9NQC7
Cellular Location:Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, centrosome, cilium basal body, cytoskeleton, microtubule organizing center, perinuclear region, spindle
Calculated MW:107kDa
Observed MW:107KDa
Synonyms:CYLD, BRSS, CDMT, CYLD1, CYLDI, EAC, MFT, MFT1, SBS, TEM, USPL2
Background:This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
UniProt Protein Function:CYLD: Protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Has endodeubiquitinase activity. Plays an important role in the regulation of pathways leading to NF-kappa-B activation. Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. Negative regulator of Wnt signaling. Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. Required for normal cell cycle progress and normal cytokinesis. Inhibits nuclear translocation of NF-kappa-B. Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. Negatively regulates TNFRSF11A signaling and osteoclastogenesis. Interacts (via CAP-Gly domain) with IKBKG/NEMO (via proline-rich C-terminal region). Interacts with TRAF2 and TRIP. Interacts with PLK1, DVL1, DVL3, MAVS, TBK1, IKKE and DDX58. Interacts (via CAP-Gly domain) with microtubules. Interacts with HDAC6 and BCL3. Interacts with SQSTM1 and MAP3K7. Identified in a complex with TRAF6 and SQSTM1. Detected in fetal brain, testis, and skeletal muscle, and at a lower level in adult brain, leukocytes, liver, heart, kidney, spleen, ovary and lung. Isoform 2 is found in all tissues except kidney. Inhibited by phosphorylation at serine residues. Belongs to the peptidase C67 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin-specific protease; Protease; Tumor suppressor; EC 3.4.19.12; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: nucleoplasm; centrosome; extrinsic to internal side of plasma membrane; perinuclear region of cytoplasm; cytoplasmic microtubule; spindle; midbody; cytosol

Molecular Function:protein binding; structural constituent of ribosome; zinc ion binding; ubiquitin-specific protease activity; protein kinase binding

Biological Process: ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway; inhibition of NF-kappaB transcription factor; regulation of mitotic cell cycle; innate immune response; negative regulation of NF-kappaB import into nucleus; cell cycle; negative regulation of interferon type I production; negative regulation of T cell differentiation

Disease: Brooke-spiegler Syndrome; Cylindromatosis, Familial; Trichoepithelioma, Multiple Familial, 1

NCBI Summary:This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:Q9NQC7
NCBI GenInfo Identifier:51316104
NCBI Gene ID:1540
NCBI Accession:Q9NQC7.1
UniProt Related Accession:Q9NQC7
Molecular Weight:120kDa
NCBI Full Name:Ubiquitin carboxyl-terminal hydrolase CYLD
NCBI Synonym Full Names:CYLD lysine 63 deubiquitinase
NCBI Official Symbol:CYLD  
NCBI Official Synonym Symbols:EAC; MFT; SBS; TEM; BRSS; CDMT; MFT1; CYLD1; CYLDI; USPL2  
NCBI Protein Information:ubiquitin carboxyl-terminal hydrolase CYLD
UniProt Protein Name:Ubiquitin carboxyl-terminal hydrolase CYLD
UniProt Synonym Protein Names:Deubiquitinating enzyme CYLD; Ubiquitin thioesterase CYLD; Ubiquitin-specific-processing protease CYLD
Protein Family:Ubiquitin carboxyl-terminal hydrolase
UniProt Gene Name:CYLD  
UniProt Entry Name:CYLD_HUMAN
Anti-CYLD Antibody (CAB3821)Western blot analysis of extracts of various cell lines, using CYLD antibody (CAB3821) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CYLD Antibody (CAB3821)Immunohistochemistry of paraffin-embedded rat brain using CYLD Rabbit pAb (CAB3821) at dilution of 1:100 (40x lens).
Anti-CYLD Antibody (CAB3821)Immunohistochemistry of paraffin-embedded rat spleen using CYLD Rabbit pAb (CAB3821) at dilution of 1:100 (40x lens).
Anti-CYLD Antibody (CAB3821)Immunohistochemistry of paraffin-embedded human lung cancer using CYLD Rabbit pAb (CAB3821) at dilution of 1:100 (40x lens).
Anti-CYLD Antibody (CAB3821)Immunohistochemistry of paraffin-embedded human esophageal cancer using CYLD Rabbit pAb (CAB3821) at dilution of 1:100 (40x lens).

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