Anti-Cyclin B1 Antibody (CAB2056)
- SKU:
- CAB2056
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-Cyclin B1 Antibody |
| Antibody SKU: | CAB2056 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 330 to the C-terminus of human Cyclin B1 (NP_114172.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, PC-3 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 330 to the C-terminus of human Cyclin B1 (NP_114172.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TMLD YDMV HFPP SQIA AGAF CLAL KILD NGEW TPTL QHYL SYTE ESLL PVMQ HLAK NVVM VNQG LTKH MTVK NKYA TSKH AKIS TLPQ LNSA LVQD LAKA VAKV |
| Gene ID: | 891 |
| Uniprot: | P14635 |
| Cellular Location: | Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center |
| Calculated MW: | 44kDa/48kDa |
| Observed MW: | 55kDa |
| Synonyms: | CCNB, CCNB1, Cyclin B1, cyclin B1 |
| Background: | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. |
| UniProt Protein Function: | CCNB1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Complexes with cdc2 to form the maturation-promoting factor (MPF). Two alternatively spliced isoforms have been found, a constitutively expressed form and a cell cycle-regulated form that is expressed predominantly during G2/M phase. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Activator Chromosomal Location of Human Ortholog: 5q12 Cellular Component: nucleoplasm; spindle pole; centrosome; membrane; cytoplasm; nucleus; cytosol Molecular Function:protein binding; patched binding; histone kinase activity; protein kinase binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of histone phosphorylation; regulation of cell cycle; ventricular cardiac muscle cell development; positive regulation of attachment of spindle microtubules to kinetochore; mitotic nuclear envelope disassembly; positive regulation of mRNA 3'-end processing; positive regulation of mitotic cell cycle; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; tissue regeneration; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; protein complex assembly; G2/M transition of mitotic cell cycle; positive regulation of cardiac muscle cell proliferation; response to drug; in utero embryonic development; oocyte maturation; mitotic metaphase plate congression; response to DDT; gut development; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; response to mechanical stimulus; cell division; spermatogenesis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; mitotic spindle stabilization; G1/S transition of mitotic cell cycle |
| NCBI Summary: | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P14635 |
| NCBI GenInfo Identifier: | 116176 |
| NCBI Gene ID: | 891 |
| NCBI Accession: | P14635.1 |
| UniProt Secondary Accession: | P14635,Q5TZP9, A8K066, |
| UniProt Related Accession: | P14635 |
| Molecular Weight: | 44,102 Da |
| NCBI Full Name: | G2/mitotic-specific cyclin-B1 |
| NCBI Synonym Full Names: | cyclin B1 |
| NCBI Official Symbol: | CCNB1 |
| NCBI Official Synonym Symbols: | CCNB |
| NCBI Protein Information: | G2/mitotic-specific cyclin-B1; G2/mitotic-specific cyclin B1 |
| UniProt Protein Name: | G2/mitotic-specific cyclin-B1 |
| UniProt Gene Name: | CCNB1 |
| UniProt Entry Name: | CCNB1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Cyclin B1 Antibody (CAB2056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). |
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