Anti-Cyclin A2 Antibody (CAB7632)
- SKU:
- CAB7632
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-Cyclin A2 Antibody |
| Antibody SKU: | CAB7632 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human Cyclin A2 |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain, Mouse liver, Mouse spleen, Mouse eye, Rat spinal cord |
| Immunogen: | A synthetic peptide of human Cyclin A2 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 890 |
| Uniprot: | P20248 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 48kDa |
| Observed MW: | 55kDa |
| Synonyms: | CCN1, CCNA, Cyclin A2, CCNA2, cyclin-A2 |
| Background: | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. |
| UniProt Protein Function: | CCNA2: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 4q27 Cellular Component: nucleoplasm; male pronucleus; cytoplasm; female pronucleus; nucleus Molecular Function:protein binding; protein kinase binding Biological Process: mitosis; positive regulation of fibroblast proliferation; organ regeneration; response to glucagon stimulus; cell division; positive regulation of transcription, DNA-dependent; mitotic cell cycle G2/M transition DNA damage checkpoint; regulation of G2/M transition of mitotic cell cycle; Ras protein signal transduction; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; response to estradiol stimulus |
| NCBI Summary: | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M. [provided by RefSeq, Aug 2016] |
| UniProt Code: | P20248 |
| NCBI GenInfo Identifier: | 311033358 |
| NCBI Gene ID: | 890 |
| NCBI Accession: | P20248.2 |
| UniProt Related Accession: | P20248 |
| Molecular Weight: | ~ 49kDa |
| NCBI Full Name: | Cyclin-A2 |
| NCBI Synonym Full Names: | cyclin A2 |
| NCBI Official Symbol: | CCNA2 |
| NCBI Official Synonym Symbols: | CCN1; CCNA |
| NCBI Protein Information: | cyclin-A2 |
| UniProt Protein Name: | Cyclin-A2 |
| Protein Family: | Protein |
| UniProt Gene Name: | CCNA2 |
| UniProt Entry Name: | CCNA2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody (CAB7632) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using Cyclin A2 antibody (CAB7632) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using Cyclin A2 antibody (CAB7632) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using Cyclin A2 antibody (CAB7632) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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