Anti-Cyclin A1 Antibody (CAB14529)
- SKU:
- CAB14529
- Product type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-Cyclin A1 Antibody |
| Antibody SKU: | CAB14529 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 350-450 of human CCNA1 (NP_001104515.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse brain, Mouse testis, Mouse heart, Rat brain, Rat testis, Rat heart |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 350-450 of human CCNA1 (NP_001104515.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YLRR QGVC VRTE NLAK YVAE LSLL EADP FLKY LPSL IAAA AFCL ANYT VNKH FWPE TLAA FTGY SLSE IVPC LSEL HKAY LDIP HRPQ QAIR EKYK ASKY L |
| Gene ID: | 8900 |
| Uniprot: | P78396 |
| Cellular Location: | Nucleus |
| Calculated MW: | 47kDa/52kDa |
| Observed MW: | 52kDa |
| Synonyms: | CCNA1, CT146, cyclin-A1 |
| Background: | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | CCNA1: May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. Belongs to the cyclin family. Cyclin AB subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cancer Testis Antigen (CTA); Cell cycle regulation; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 13q12.3-q13 Cellular Component: nucleoplasm; microtubule cytoskeleton; cytosol Molecular Function:protein binding; protein kinase binding Biological Process: G1/S-specific transcription in mitotic cell cycle; mitosis; male meiosis I; cell division; regulation of G2/M transition of mitotic cell cycle; spermatogenesis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle |
| NCBI Summary: | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P78396 |
| NCBI GenInfo Identifier: | 8134359 |
| NCBI Gene ID: | 8900 |
| NCBI Accession: | P78396.1 |
| UniProt Secondary Accession: | P78396,Q5T3V0, Q5U0G2, Q8IY91, B7Z7E3, |
| UniProt Related Accession: | P78396 |
| Molecular Weight: | 465 |
| NCBI Full Name: | Cyclin-A1 |
| NCBI Synonym Full Names: | cyclin A1 |
| NCBI Official Symbol: | CCNA1 |
| NCBI Official Synonym Symbols: | CT146 |
| NCBI Protein Information: | cyclin-A1 |
| UniProt Protein Name: | Cyclin-A1 |
| Protein Family: | Cyclin |
| UniProt Gene Name: | CCNA1 |
| UniProt Entry Name: | CCNA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Cyclin A1 antibody (CAB14529) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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