Anti-CYB5R3 Antibody (CAB7535)
- SKU:
- CAB7535
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-CYB5R3 Antibody |
| Antibody SKU: | CAB7535 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 125-334 of human CYB5R3 (NP_001165131.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, HeLa, HT-1080, NIH/3T3, Mouse kidney, Mouse brain, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 125-334 of human CYB5R3 (NP_001165131.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RPYT PISS DDDK GFVD LVIK VYFK DTHP KFPA GGKM SQYL ESMQ IGDT IEFR GPSG LLVY QGKG KFAI RPDK KSNP IIRT VKSV GMIA GGTG ITPM LQVI RAIM KDPD DHTV CHLL FANQ TEKD ILLR PELE ELRN KHSA RFKL WYTL DRAP EAWD YGQG FVNE EMIR DHLP PPEE EPLV LMCG PPPM IQYA CLPN LDHV GHPT ERCF VF |
| Gene ID: | 1727 |
| Uniprot: | P00387 |
| Cellular Location: | Cytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Lipid-anchor, Mitochondrion outer membrane |
| Calculated MW: | 31kDa/34kDa/38kDa |
| Observed MW: | 34-36kDa |
| Synonyms: | CYB5R3, B5R, DIA1 |
| Background: | This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. |
| UniProt Protein Function: | CYB5R3: Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Defects in CYB5R3 are the cause of methemoglobinemia CYB5R3-related (METHB-CYB5R3). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. There are two types of methemoglobinemia CYB5R3-related. In type 1, the defect affects the soluble form of the enzyme, is restricted to red blood cells, and causes well- tolerated methemoglobinemia. In type 2, the defect affects both the soluble and microsomal forms of the enzyme and is thus generalized, affecting red cells, leukocytes and all body tissues. Type 2 methemoglobinemia is associated with mental deficiency and other neurologic symptoms. Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. 3 isoforms of the human protein are produced by alternative promoter. |
| UniProt Protein Details: | Protein type:EC 1.6.2.2; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Oxidoreductase; Mitochondrial Chromosomal Location of Human Ortholog: 22q13.2 Cellular Component: mitochondrial outer membrane; endoplasmic reticulum membrane; mitochondrion; membrane; endoplasmic reticulum; hemoglobin complex; cytoplasm; mitochondrial inner membrane; lipid particle Molecular Function:FAD binding; cytochrome-b5 reductase activity; ADP binding; NAD binding; AMP binding Biological Process: vitamin metabolic process; blood circulation; L-ascorbic acid metabolic process; cholesterol biosynthetic process; water-soluble vitamin metabolic process Disease: Methemoglobinemia Due To Deficiency Of Methemoglobin Reductase |
| NCBI Summary: | This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq, Jan 2010] |
| UniProt Code: | P00387 |
| NCBI GenInfo Identifier: | 127846 |
| NCBI Gene ID: | 1727 |
| NCBI Accession: | P00387.3 |
| UniProt Secondary Accession: | P00387,O75675, Q8TDL8, Q8WTS8, Q9UEN4, Q9UEN5, Q9UL55 Q9UL56, B1AHF2, B7Z7L3, |
| UniProt Related Accession: | P00387 |
| Molecular Weight: | 38,358 Da |
| NCBI Full Name: | NADH-cytochrome b5 reductase 3 |
| NCBI Synonym Full Names: | cytochrome b5 reductase 3 |
| NCBI Official Symbol: | CYB5R3 |
| NCBI Official Synonym Symbols: | B5R; DIA1 |
| NCBI Protein Information: | NADH-cytochrome b5 reductase 3; diaphorase-1; NADH-cytochrome b5 reductase 3 soluble form; NADH-cytochrome b5 reductase 3 membrane-bound form |
| UniProt Protein Name: | NADH-cytochrome b5 reductase 3 |
| UniProt Synonym Protein Names: | Diaphorase-1Cleaved into the following 2 chains:NADH-cytochrome b5 reductase 3 membrane-bound form; NADH-cytochrome b5 reductase 3 soluble form |
| Protein Family: | NADH-cytochrome b5 reductase |
| UniProt Gene Name: | CYB5R3 |
| UniProt Entry Name: | NB5R3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CYB5R3 antibody (CAB7535) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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