Anti-CX3CL1 항체 (CAB14198)

Antibody Name:	Anti-CX3CL1 Antibody
Antibody SKU:	CAB14198
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 365-397aa of human CX3CL1 (NP_002987.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 365-397aa of human CX3CL1 (NP_002987.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	6376
Uniprot:	P78423
Cellular Location:	Cell membrane, Secreted, Single-pass type I membrane protein
Calculated MW:	42kDa
Observed MW:	100KDa

Synonyms:

CX3CL1, ABCD-3, C3Xkine, CXC3, CXC3C, NTN, NTT, SCYD1, fractalkine

Background:

This gene belongs to the CX3C subgroup of chemokines, characterized by the number of amino acids located between the conserved cysteine residues. This is the only member of the CX3C subgroup, which contains three amino acids between cysteine residues, resulting in a Cys-X-X-X-Cys configuration. The encoded protein contains an extended mucin-like stalk with a chemokine domain on top, and exists in both a membrane-anchored form where it acts as a binding molecule, or, in soluble form, as a chemotactic cytokine. The mature form of this protein can be cleaved at the cell surface, yielding different soluble forms that can interact with the G-protein coupled receptor, C-X3-C motif chemokine receptor 1 gene product. This gene plays a role in a wide range of diseases, including cancer, vasculitis, neuropathies, atherosclerosis, inflammatory diseases, and in human immunodeficiency virus infections.

UniProt Protein Function:	CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Membrane protein, integral Chromosomal Location of Human Ortholog: 16q13 Cellular Component: extracellular space; cell surface; integral to membrane; plasma membrane; extracellular region Molecular Function:protein binding; chemokine activity; receptor binding Biological Process: positive regulation of transforming growth factor-beta1 production; neutrophil chemotaxis; leukocyte chemotaxis; cytokine and chemokine mediated signaling pathway; defense response; chemotaxis; angiogenesis involved in wound healing; macrophage chemotaxis; leukocyte adhesive activation; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; immune response; cell adhesion; lymphocyte chemotaxis; positive regulation of inflammatory response
UniProt Code:	P78423
NCBI GenInfo Identifier:	6175080
NCBI Gene ID:	6376
NCBI Accession:	P78423.1
UniProt Secondary Accession:	P78423,O00672,
UniProt Related Accession:	P78423
Molecular Weight:	42,203 Da
NCBI Full Name:	Fractalkine
NCBI Synonym Full Names:	chemokine (C-X3-C motif) ligand 1
NCBI Official Symbol:	CX3CL1
NCBI Official Synonym Symbols:	NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
NCBI Protein Information:	fractalkine; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)
UniProt Protein Name:	Fractalkine
UniProt Synonym Protein Names:	C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1Cleaved into the following chain:Processed fractalkine
Protein Family:	Fractalkine
UniProt Gene Name:	CX3CL1
UniProt Entry Name:	X3CL1_HUMAN

	Western blot analysis of extracts of Mouse skeletal muscle, using CX3CL1 antibody (CAB14198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts of SH-SY5Y cells, using CX3CL1 antibody (CAB14198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human brain using CX3CL1 Rabbit pAb (CAB14198) at dilution of 1:150 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using CX3CL1 Rabbit pAb (CAB14198) at dilution of 1:150 (40x lens).
	Immunohistochemistry of paraffin-embedded rat ovary using CX3CL1 Rabbit pAb (CAB14198) at dilution of 1:150 (40x lens).

Antibodies	ELISA Kits
CX3CL1 Antibody (PACO02042)	Human CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) CLIA Kit (HUES00043)
CX3CL1 Antibody (PACO08682)	Human CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) ELISA Kit (HUES01331)
	Human Fractalkine (CX3CL1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)