Anti-CTCF Antibody (CAB18627)[KO Validated]

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SKU:
CAB18627
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Antibody Name:Anti-CTCF Antibody [KO Validated]
Antibody SKU:CAB18627
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human CTCF.
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, NIH/3T3, C6
Immunogen:Recombinant protein of human CTCF.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:10664
Uniprot:P49711
Cellular Location:Chromosome, Nucleus, centromere, nucleoplasm
Calculated MW:45kDa/82kDa
Observed MW:140kDa
Anti-CTCF Antibody [KO Validated] (CAB18627)Western blot analysis of extracts from normal (control) and CTCF knockout (KO) 293T cells, using CTCF antibody (CAB18627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-CTCF Antibody [KO Validated] (CAB18627)Western blot analysis of extracts of various cell lines, using CTCF antibody (CAB18627) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-CTCF Antibody [KO Validated] (CAB18627)Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] CTCF Rabbit pAb (CAB18627) at dilution of 1:250 (40x lens).
Anti-CTCF Antibody [KO Validated] (CAB18627)Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug CTCF antibody (CAB18627). Western blot was performed from the immunoprecipitate using CTCF antibody (CAB18627) at a dilition of 1:500.
Synonyms:CTCF, MRD21, CCCTC-binding factor
Background:This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:CTCF: a widely expressed, sequence-specific DNA-binding transcriptional regulator, insulator, and organizer of higher-order chromatin structure. Acts as a tumor suppressor. Contains 11 C2H2-type zinc fingers. Involved in promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Mediates pairing between X chromosomes and interactions between distant regulatory elements. Binds to promoters of c-myc, PLK, PIM1 and APP. A critical regulator of cell-cycle arrest and death after B cell receptor signaling in immature B cells. CTCF, together with YY1 and Tsix, form a regulated epigenetic switch for X-inactivation.
UniProt Protein Details:

Protein type:Tumor suppressor; C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 16q21-q22.3

Cellular Component: nucleoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region

Molecular Function:protein binding; zinc ion binding; sequence-specific DNA binding; chromatin insulator sequence binding; transcription factor activity; transcription corepressor activity

Biological Process: genetic imprinting; transcription, DNA-dependent; maintenance of DNA methylation; positive regulation of transcription, DNA-dependent; regulation of histone methylation; regulation of histone acetylation; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; regulation of gene expression, epigenetic; nucleosome positioning; chromosome segregation; regulation of molecular function, epigenetic; DNA methylation; negative regulation of transcription, DNA-dependent

Disease: Mental Retardation, Autosomal Dominant 21

NCBI Summary:This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
UniProt Code:P49711
NCBI GenInfo Identifier:1706179
NCBI Gene ID:10664
NCBI Accession:P49711.1
UniProt Related Accession:P49711
Molecular Weight:83; 84; 84kDa
NCBI Full Name:Transcriptional repressor CTCF
NCBI Synonym Full Names:CCCTC-binding factor
NCBI Official Symbol:CTCF  
NCBI Official Synonym Symbols:MRD21  
NCBI Protein Information:transcriptional repressor CTCF
UniProt Protein Name:Transcriptional repressor CTCF
UniProt Synonym Protein Names:11-zinc finger protein; CCCTC-binding factor; CTCFL paralog
Protein Family:Transcriptional repressor
UniProt Gene Name:CTCF  
UniProt Entry Name:CTCF_HUMAN

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