Anti-CS Antibody (CAB5713)
- SKU:
- CAB5713
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-CS Antibody |
| Antibody SKU: | CAB5713 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 167-466 of human CS (NP_004068.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 22Rv1, Jurkat, 293T, HepG2, MCF7, HeLa, Mouse heart, Mouse liver, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 167-466 of human CS (NP_004068.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QLSA AVTA LNSE SNFA RAYA QGIS RTKY WELI YEDS MDLI AKLP CVAA KIYR NLYR EGSG IGAI DSNL DWSH NFTN MLGY TDHQ FTEL TRLY LTIH SDHE GGNV SAHT SHLV GSAL SDPY LSFA AAMN GLAG PLHG LANQ EVLV WLTQ LQKE VGKD VSDE KLRD YIWN TLNS GRVV PGYG HAVL RKTD PRYT CQRE FALK HLPN DPMF KLVA QLYK IVPN VLLE QGKA KNPW PNVD AHSG VLLQ YYGM TEMN YYTV LFGV SRAL GVLA QLIW SRAL GFPL ERPK SMST EGLM KFVD SKSG |
| Gene ID: | 1431 |
| Uniprot: | O75390 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 51kDa |
| Observed MW: | 52kDa |
| Synonyms: | CS |
| Background: | The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. |
| UniProt Protein Function: | CS: a mitochondrial enzyme of the tricarboxylic acid cycle that converts acetyl-CoA + H2O + oxaloacetate into citrate + CoA. Found in nearly all cells capable of oxidative metabolism. |
| UniProt Protein Details: | Protein type:EC 2.3.3.1; Mitochondrial; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Carbohydrate Metabolism - citrate (TCA) cycle; Transferase Chromosomal Location of Human Ortholog: 12q13.2 Cellular Component: mitochondrion; mitochondrial matrix; nucleus Molecular Function:citrate (Si)-synthase activity Biological Process: cellular metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; cellular carbohydrate metabolic process |
| NCBI Summary: | The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O75390 |
| NCBI GenInfo Identifier: | 57015285 |
| NCBI Gene ID: | 1431 |
| NCBI Accession: | O75390.2 |
| UniProt Secondary Accession: | O75390,Q71UT9, Q7KZH0, Q96FZ8, Q9BWN8, |
| UniProt Related Accession: | O75390 |
| Molecular Weight: | 466 |
| NCBI Full Name: | Citrate synthase, mitochondrial |
| NCBI Synonym Full Names: | citrate synthase |
| NCBI Official Symbol: | CS |
| NCBI Protein Information: | citrate synthase, mitochondrial; citrate (Si)-synthase |
| UniProt Protein Name: | Citrate synthase, mitochondrial |
| UniProt Synonym Protein Names: | Citrate (Si)-synthase |
| Protein Family: | Css54 |
| UniProt Gene Name: | CS |
| UniProt Entry Name: | CISY_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CS antibody (CAB5713) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 15s. |
 | Immunofluorescence analysis of MCF-7 cells using CS antibody (CAB5713). Blue: DAPI for nuclear staining. |
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
Anti-CHD1L Antibody (CAB17558)
