Anti-CRK Antibody (CAB1604)
- SKU:
- CAB1604
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-CRK Antibody |
| Antibody SKU: | CAB1604 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-304 of human CRK (NP_058431.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-549, MCF7, BT-474, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-304 of human CRK (NP_058431.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAGN FDSE ERSS WYWG RLSR QEAV ALLQ GQRH GVFL VRDS STSP GDYV LSVS ENSR VSHY IINS SGPR PPVP PSPA QPPP GVSP SRLR IGDQ EFDS LPAL LEFY KIHY LDTT TLIE PVSR SRQG SGVI LRQE EAEY VRAL FDFN GNDE EDLP FKKG DILR IRDK PEEQ WWNA EDSE GKRG MIPV PYVE KYRP ASAS VSAL IGGN QEGS HPQP LGGP EPGP YAQP SVNT PLPN LQNG PIYA RVIQ KRVP NAYD KTAL ALEV GELV KVTK INVS GQWE GECN GKRG HFPF THVR LLDQ QNPD EDFS |
| Gene ID: | 1398 |
| Uniprot: | P46108 |
| Cellular Location: | Cell membrane, Cytoplasm |
| Calculated MW: | 22kDa/33kDa |
| Observed MW: | 37kDa |
| Synonyms: | CRK, CRKII, p38 |
| Background: | This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. |
| UniProt Protein Function: | CRK: an adaptor protein with an SH2-SH3-SH3 domain structure. Recruits cytoplasmic proteins through SH2-phospho-tyrosine interaction. Phosphorylated by Abl, IGF-IR and EGFR. Phosphorylation induces a change in intramolecular folding and SH2 interactions, causing its rapid dissociation from the tyrosine kinase complex. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold; Oncoprotein Chromosomal Location of Human Ortholog: 17p13.3 Cellular Component: cytoplasm; plasma membrane; cytosol; nucleus; actin cytoskeleton Molecular Function:protein phosphorylated amino acid binding; protein binding; ephrin receptor binding; SH3/SH2 adaptor activity; SH2 domain binding Biological Process: regulation of transcription from RNA polymerase II promoter; regulation of Rac protein signal transduction; platelet activation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; regulation of actin cytoskeleton organization and biogenesis; insulin receptor signaling pathway; ephrin receptor signaling pathway; innate immune response; vascular endothelial growth factor receptor signaling pathway; blood coagulation |
| NCBI Summary: | This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P46108 |
| NCBI GenInfo Identifier: | 158939322 |
| NCBI Gene ID: | 1398 |
| NCBI Accession: | P46108.2 |
| UniProt Secondary Accession: | P46108,Q96GA9, Q96HJ0, A8MWE8, B0LPE8, D3DTH6, |
| UniProt Related Accession: | P46108 |
| Molecular Weight: | 304 |
| NCBI Full Name: | Adapter molecule crk |
| NCBI Synonym Full Names: | v-crk avian sarcoma virus CT10 oncogene homolog |
| NCBI Official Symbol: | CRK |
| NCBI Official Synonym Symbols: | p38; CRKII |
| NCBI Protein Information: | adapter molecule crk; proto-oncogene c-Crk |
| UniProt Protein Name: | Adapter molecule crk |
| UniProt Synonym Protein Names: | Proto-oncogene c-Crk; p38 |
| Protein Family: | Crk-like protein |
| UniProt Gene Name: | CRK |
| UniProt Entry Name: | CRK_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CRK antibody (CAB1604) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using CRK antibody (CAB1604) at dilution of 1:200 (40x lens). |
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