Anti-CPE Antibody (CAB5458)
- SKU:
- CAB5458
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-CPE Antibody |
| Antibody SKU: | CAB5458 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 257-476 of human CPE (NP_001864.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-549, MCF7, Mouse brain, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 257-476 of human CPE (NP_001864.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PYDE TRSG SAHE YSSS PDDA IFQS LARA YSSF NPAM SDPN RPPC RKND DDSS FVDG TTNG GAWY SVPG GMQD FNYL SSNC FEIT VELS CEKF PPEE TLKT YWED NKNS LISY LEQI HRGV KGFV RDLQ GNPI ANAT ISVE GIDH DVTS AKDG DYWR LLIP GNYK LTAS APGY LAIT KKVA VPYS PAAG VDFE LESF SERK EEEK EELM EWWK MMSE TLNF |
| Gene ID: | 1363 |
| Uniprot: | P16870 |
| Cellular Location: | Cytoplasmic vesicle, Nucleus, Peripheral membrane protein, Secreted, secretory vesicle membrane |
| Calculated MW: | 49kDa/53kDa |
| Observed MW: | 53-55kDa |
| Synonyms: | CPE, CPH |
| Background: | This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes. |
| UniProt Protein Function: | CPE: Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protease; EC 3.4.17.10; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 4q32.3 Cellular Component: cell soma; extracellular space; Golgi apparatus; plasma membrane; secretory granule; secretory granule membrane Molecular Function:carboxypeptidase activity; cell adhesion molecule binding; metallocarboxypeptidase activity; neurexin binding; serine carboxypeptidase activity Biological Process: insulin processing; metabolic process; Wnt receptor signaling pathway |
| NCBI Summary: | This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes. [provided by RefSeq, Nov 2015] |
| UniProt Code: | P16870 |
| NCBI GenInfo Identifier: | 115892 |
| NCBI Gene ID: | 1363 |
| NCBI Accession: | P16870.1 |
| UniProt Secondary Accession: | P16870,Q9UIU9, A8K4N1, B3KR42, B4DFN4, D3DP33, |
| UniProt Related Accession: | P16870 |
| Molecular Weight: | 49,886 Da |
| NCBI Full Name: | Carboxypeptidase E |
| NCBI Synonym Full Names: | carboxypeptidase E |
| NCBI Official Symbol: | CPE |
| NCBI Official Synonym Symbols: | CPH |
| NCBI Protein Information: | carboxypeptidase E |
| UniProt Protein Name: | Carboxypeptidase E |
| UniProt Synonym Protein Names: | Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase |
| Protein Family: | Carboxypeptidase |
| UniProt Gene Name: | CPE |
| UniProt Entry Name: | CBPE_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CPE antibody (CAB5458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of U2OS cells using CPE antibody (CAB5458). Blue: DAPI for nuclear staining. |
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