Anti-COX6B1 항체 (CAB2641)

Antibody Name:	Anti-COX6B1 Antibody
Antibody SKU:	CAB2641
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-86 of human COX6B1 (NP_001854.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	A-549, MCF7, Jurkat, HepG2, Mouse brain, Mouse small intestine, Mouse liver, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-86 of human COX6B1 (NP_001854.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAED METK IKNY KTAP FDSR FPNQ NQTR NCWQ NYLD FHRC QKAM TAKG GDIS VCEW YQRV YQSL CPTS WVTD WDEQ RAEG TFPG KI
Gene ID:	1340
Uniprot:	P14854
Cellular Location:	Mitochondrion intermembrane space
Calculated MW:	10kDa
Observed MW:	13kDa

Synonyms:

COX6B1, COX6B, COXG, COXVIb1

Background:

Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes subunit VIb. Mutations in this gene are associated with severe infantile encephalomyopathy. Three pseudogenes COX6BP-1, COX6BP-2 and COX6BP-3 have been found on chromosomes 7, 17 and 22q13.1-13.2, respectively.

UniProt Protein Function:	COX6B1: Connects the two COX monomers into the physiological dimeric form. Defects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D); also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome. Belongs to the cytochrome c oxidase subunit 6B family.
UniProt Protein Details:	Protein type:Mitochondrial; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 19q13.1 Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space Molecular Function:cytochrome-c oxidase activity Biological Process: cellular metabolic process; substantia nigra development; transmembrane transport Disease: Mitochondrial Complex Iv Deficiency
NCBI Summary:	Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes subunit VIb. Mutations in this gene are associated with severe infantile encephalomyopathy. Three pseudogenes COX6BP-1, COX6BP-2 and COX6BP-3 have been found on chromosomes 7, 17 and 22q13.1-13.2, respectively. [provided by RefSeq, Jan 2010]
UniProt Code:	P14854
NCBI GenInfo Identifier:	117115
NCBI Gene ID:	1340
NCBI Accession:	P14854.2
UniProt Related Accession:	P14854
Molecular Weight:	10kDa
NCBI Full Name:	Cytochrome c oxidase subunit 6B1
NCBI Synonym Full Names:	cytochrome c oxidase subunit 6B1
NCBI Official Symbol:	COX6B1
NCBI Official Synonym Symbols:	COXG; COX6B; COXVIb1
NCBI Protein Information:	cytochrome c oxidase subunit 6B1
UniProt Protein Name:	Cytochrome c oxidase subunit 6B1
UniProt Synonym Protein Names:	Cytochrome c oxidase subunit VIb isoform 1; COX VIb-1
Protein Family:	Cytochrome c oxidase
UniProt Gene Name:	COX6B1
UniProt Entry Name:	CX6B1_HUMAN

	Western blot analysis of extracts of various cell lines, using COX6B1 antibody (CAB2641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded mouse heart using COX6B1 antibody (CAB2641) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human trachea using COX6B1 antibody (CAB2641) at dilution of 1:100 (40x lens).

Antibodies

Anti-COX6B1 Antibody (CAB12425)

COX6B1 Antibody (PACO08550)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)