Anti-COX5A Antibody (CAB6437)
- SKU:
- CAB6437
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-COX5A Antibody |
| Antibody SKU: | CAB6437 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human COX5A (NP_004246.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, mouse skeletal muscle, mouse testis, mouse heart, mouse brain, rat heart, rat kidney, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human COX5A (NP_004246.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLGA ALRR CAVA ATTR ADPR GLLH SART PGPA VAIQ SVRC YSHG SQET DEEF DARW VTYF NKPD IDAW ELRK GINT LVTY DMVP EPKI IDAA LRAC RRLN DFAS TVRI LEVV KDKA GPHK EIYP YVIQ ELRP TLNE LGIS TPEE LGLD KV |
| Gene ID: | 9377 |
| Uniprot: | P20674 |
| Cellular Location: | Mitochondrion inner membrane |
| Calculated MW: | 16kDa |
| Observed MW: | 14kDa |
| Synonyms: | COX5A, COX, COX-VA, VA |
| Background: | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. |
| UniProt Protein Function: | COX5A: This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Belongs to the cytochrome c oxidase subunit 5A family. |
| UniProt Protein Details: | Protein type:EC 1.9.3.1; Oxidoreductase; Energy Metabolism - oxidative phosphorylation; Mitochondrial Chromosomal Location of Human Ortholog: 15q24.1 Cellular Component: mitochondrial inner membrane Molecular Function:cytochrome-c oxidase activity; electron carrier activity; metal ion binding Biological Process: cellular metabolic process; transmembrane transport |
| NCBI Summary: | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P20674 |
| NCBI GenInfo Identifier: | 218511986 |
| NCBI Gene ID: | 9377 |
| NCBI Accession: | P20674.2 |
| UniProt Secondary Accession: | P20674,P30045, Q8TB65, |
| UniProt Related Accession: | P20674 |
| Molecular Weight: | 150 |
| NCBI Full Name: | Cytochrome c oxidase subunit 5A, mitochondrial |
| NCBI Synonym Full Names: | cytochrome c oxidase subunit Va |
| NCBI Official Symbol: | COX5A |
| NCBI Official Synonym Symbols: | VA; COX; COX-VA |
| NCBI Protein Information: | cytochrome c oxidase subunit 5A, mitochondrial; cytochrome c oxidase polypeptide Va; mitochondrial cytochrome c oxidase subunit Va; cytochrome c oxidase polypeptide, mitochondrial |
| UniProt Protein Name: | Cytochrome c oxidase subunit 5A, mitochondrial |
| UniProt Synonym Protein Names: | Cytochrome c oxidase polypeptide Va |
| Protein Family: | Cytochrome c oxidase |
| UniProt Gene Name: | COX5A |
| UniProt Entry Name: | COX5A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using COX5A Antibody (CAB6437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat ovary using COX5A antibody (CAB6437) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using COX5A antibody (CAB6437) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using COX5A antibody (CAB6437) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using COX5A antibody (CAB6437). Blue: DAPI for nuclear staining. |
Related Products
Human COX5A ELISA Kit
