Anti-COX IV Antibody (CAB6564)
- SKU:
- CAB6564
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-COX IV Antibody |
| Antibody SKU: | CAB6564 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-169 of human COX IV (NP_001852.1). |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 22Rv1, Hep G2, 293T, Mouse Kidney, Mouse heart, Mouse brain, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-169 of human COX IV (NP_001852.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AHES VVKS EDFS LPAY MDRR DHPL PEVA HVKH LSAS QKAL KEKE KASW SSLS MDEK VELY RIKF KESF AEMN RGSN EWKT VVGG AMFF IGFT ALVI MWQK HYVY GPLP QSFD KEWV AKQT KRML DMKV NPIQ GLAS KWDY EKNE WKK |
| Gene ID: | 1327 |
| Uniprot: | P13073 |
| Cellular Location: | Mitochondrion inner membrane |
| Calculated MW: | 19kDa |
| Observed MW: | 17kDa |
| Synonyms: | COX4I1, COX IV-1, COX4, COX4-1, COXIV, COXIV-1, COX IV |
| Background: | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | COX4I1: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Ubiquitous. Belongs to the cytochrome c oxidase IV family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Energy Metabolism - oxidative phosphorylation; Mitochondrial; EC 1.9.3.1; Oxidoreductase Chromosomal Location of Human Ortholog: 16q24.1 Cellular Component: membrane; mitochondrial inner membrane; mitochondrial respiratory chain complex IV; mitochondrion; nucleus Molecular Function:protein binding Biological Process: generation of precursor metabolites and energy; mitochondrial electron transport, cytochrome c to oxygen |
| NCBI Summary: | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] |
| UniProt Code: | P13073 |
| NCBI GenInfo Identifier: | 117086 |
| NCBI Gene ID: | 1327 |
| NCBI Accession: | P13073.1 |
| UniProt Secondary Accession: | P13073,Q6P666, B2R4J2, D3DUM7, |
| UniProt Related Accession: | P13073 |
| Molecular Weight: | 19,577 Da |
| NCBI Full Name: | Cytochrome c oxidase subunit 4 isoform 1, mitochondrial |
| NCBI Synonym Full Names: | cytochrome c oxidase subunit 4I1 |
| NCBI Official Symbol: | COX4I1 |
| NCBI Official Synonym Symbols: | COX4; COXIV; COX4-1; COXIV-1; COX IV-1 |
| NCBI Protein Information: | cytochrome c oxidase subunit 4 isoform 1, mitochondrial |
| UniProt Protein Name: | Cytochrome c oxidase subunit 4 isoform 1, mitochondrial |
| UniProt Synonym Protein Names: | Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1 |
| Protein Family: | Cytochrome c oxidase |
| UniProt Gene Name: | COX4I1 |
| UniProt Entry Name: | COX41_HUMAN |
 | Western blot analysis of extracts of various cell lines, using COX IV Antibody (CAB6564) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunofluorescence analysis of U2OS cells using COX IV antibody (CAB6564). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of 22Rv1 cells, using 3 ug COX IV antibody (CAB6564). Western blot was performed from the immunoprecipitate using COX IV antibody (CAB6564) at a dilition of 1:1000. |
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