Anti-COPE 항체 (CAB10047)

Antibody Name:	Anti-COPE Antibody
Antibody SKU:	CAB10047
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-308 of human COPE (NP_009194.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	SH-SY5Y, 293T, HeLa, Raji, LO2, Mouse pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-308 of human COPE (NP_009194.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAPP APGP ASGG SGEV DELF DVKN AFYI GSYQ QCIN EAQR VKLS SPER DVER DVFL YRAY LAQR KFGV VLDE IKPS SAPE LQAV RMFA DYLA HESR RDSI VAEL DREM SRSV DVTN TTFL LMAA SIYL HDQN PDAA LRAL HQGD SLEC TAMT VQIL LKLD RLDL ARKE LKRM QDLD EDAT LTQL ATAW VSLA TGGE KLQD AYYI FQEM ADKC SPTL LLLN GQAA CHMA QGRW EAAE GLLQ EALD KDSG YPET LVNL IVLS QHLG KPPE VTNR YLSQ LKDA HRSH PFIK EYQA KEND FDRL VLQY APSA
Gene ID:	11316
Uniprot:	O14579
Cellular Location:	COPI-coated vesicle membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus membrane, Peripheral membrane protein
Calculated MW:	28kDa/34kDa
Observed MW:	34kDa

Synonyms:

COPE, epsilon-COP

Background:

The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	COPE: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Belongs to the COPE family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Vesicle Chromosomal Location of Human Ortholog: 19p13.11 Cellular Component: COPI vesicle coat; cytosol; endoplasmic reticulum membrane; Golgi apparatus; Golgi membrane; nucleoplasm; transport vesicle Molecular Function:protein binding Biological Process: ER to Golgi vesicle-mediated transport; intra-Golgi vesicle-mediated transport; retrograde vesicle-mediated transport, Golgi to ER
NCBI Summary:	The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	O14579
NCBI GenInfo Identifier:	6647482
NCBI Gene ID:	11316
NCBI Accession:	O14579.3
UniProt Secondary Accession:	O14579,O76097, Q6IBB8, Q9UGP6, A6NE29, A6NKA3,
UniProt Related Accession:	O14579
Molecular Weight:	28,773 Da
NCBI Full Name:	Coatomer subunit epsilon
NCBI Synonym Full Names:	coatomer protein complex subunit epsilon
NCBI Official Symbol:	COPE
NCBI Official Synonym Symbols:	epsilon-COP
NCBI Protein Information:	coatomer subunit epsilon
UniProt Protein Name:	Coatomer subunit epsilon
UniProt Synonym Protein Names:	Epsilon-coat protein; Epsilon-COP
Protein Family:	Coatomer
UniProt Gene Name:	COPE
UniProt Entry Name:	COPE_HUMAN

Western blot analysis of extracts of various cell lines, using COPE antibody (CAB10047) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

COPE Antibody (PACO08521)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)