Anti-Complement factor D Antibody (CAB8117)
- SKU:
- CAB8117
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| Antibody Name: | CFD Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8117 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-253 of human CFD (NP_001919.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-549, 22Rv1, THP-1, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-253 of human CFD (NP_001919.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ILGG REAE AHAR PYMA SVQL NGAH LCGG VLVA EQWV LSAA HCLE DAAD GKVQ VLLG AHSL SQPE PSKR LYDV LRAV PHPD SQPD TIDH DLLL LQLS EKAT LGPA VRPL PWQR VDRD VAPG TLCD VAGW GIVN HAGR RPDS LQHV LLPV LDRA TCNR RTHH DGAI TERL MCAE SNRR DSCK GDSG GPLV CGGV LEGV VTSG SRVC GNRK KPGI YTRV ASYA AWID SVLA |
| Gene ID: | 1675 |
| Uniprot: | P00746 |
| Cellular Location: | Secreted |
| Calculated MW: | 27kDa |
| Observed MW: | 27kDa |
| Synonyms: | CFD, ADIPSIN, ADN, DF, PFD |
| Background: | This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease. |
| UniProt Protein Function: | CFD: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Defects in CFD are the cause of complement factor D deficiency (CFDD). CFDD is an immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Belongs to the peptidase S1 family. |
| UniProt Protein Details: | Protein type:Secreted; EC 3.4.21.46; Secreted, signal peptide; Protease Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: extracellular region Molecular Function:serine-type endopeptidase activity; serine-type peptidase activity Biological Process: complement activation; complement activation, alternative pathway; platelet degranulation Disease: Complement Factor D Deficiency |
| NCBI Summary: | This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease. [provided by RefSeq, Nov 2015] |
| UniProt Code: | P00746 |
| NCBI GenInfo Identifier: | 158515408 |
| NCBI Gene ID: | 1675 |
| NCBI Accession: | P00746.5 |
| UniProt Secondary Accession: | P00746,Q5U5S1, Q86VJ5, Q8N4E0, Q8WZB4, B4DV76, |
| UniProt Related Accession: | P00746 |
| Molecular Weight: | 27,033 Da |
| NCBI Full Name: | Complement factor D |
| NCBI Synonym Full Names: | complement factor D |
| NCBI Official Symbol: | CFD |
| NCBI Official Synonym Symbols: | DF; ADN; PFD; ADIPSIN |
| NCBI Protein Information: | complement factor D |
| UniProt Protein Name: | Complement factor D |
| UniProt Synonym Protein Names: | Adipsin; C3 convertase activator; Properdin factor D |
| Protein Family: | Complement factor |
| UniProt Gene Name: | CFD |
| UniProt Entry Name: | CFAD_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CFD antibody (CAB8117) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 20s. |
 | Immunohistochemistry of paraffin-embedded human prostate using CFD antibody (CAB8117) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using CFD antibody (CAB8117) at dilution of 1:100 (40x lens). |
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