Anti-Complement Factor B Antibody (CAB1706)
- SKU:
- CAB1706
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| Antibody Name: | Anti-Complement Factor B Antibody |
| Antibody SKU: | CAB1706 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-420 of human Complement Factor B (NP_001701.2). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:20 - 1:50 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, K-562, 293T, A-549, HepG2, Mouse liver, Rat liver, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-420 of human Complement Factor B (NP_001701.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GSWS TLKT QDQK TVRK AECR AIHC PRPH DFEN GEYW PRSP YYNV SDEI SFHC YDGY TLRG SANR TCQV NGRW SGQT AICD NGAG YCSN PGIP IGTR KVGS QYRL EDSV TYHC SRGL TLRG SQRR TCQE GGSW SGTE PSCQ DSFM YDTP QEVA EAFL SSLT ETIE GVDA EDGH GPGE QQKR KIVL DPSG SMNI YLVL DGSD SIGA SNFT GAKK CLVN LIEK VASY GVKP RYGL VTYA TYPK IWVK VSEA DSSN ADWV TKQL NEIN YEDH KLKS GTNT KKAL QAVY SMMS WPDD VPPE GWNR TRHV IILM TDGL HNMG GDPI TVID EIRD LLYI GKDR KNPR E |
| Gene ID: | 629 |
| Uniprot: | P00751 |
| Cellular Location: | Secreted |
| Calculated MW: | 68kDa/85kDa |
| Observed MW: | 86kDa |
| Synonyms: | CFB, AHUS4, ARMD14, BF, BFD, CFAB, CFBD, FB, FBI12, GBG, H2-Bf, PBF2 |
| Background: | This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. |
| UniProt Protein Function: | CFB: Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B- lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. Defects in CFB are a cause of susceptibility to hemolytic uremic syndrome atypical type 4 (AHUS4). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protease; Secreted, signal peptide; EC 3.4.21.47; Secreted Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: extracellular region; extracellular space; plasma membrane Molecular Function:complement binding; serine-type endopeptidase activity Biological Process: complement activation; complement activation, alternative pathway; regulation of complement activation Disease: Complement Factor B Deficiency; Hemolytic Uremic Syndrome, Atypical, Susceptibility To, 4; Macular Degeneration, Age-related, 14 |
| NCBI Summary: | This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P00751 |
| NCBI GenInfo Identifier: | 584908 |
| NCBI Gene ID: | 629 |
| NCBI Accession: | P00751.2 |
| UniProt Secondary Accession: | P00751,O15006, Q29944, Q53F89, Q5JP67, Q5ST50, Q96HX6 Q9BTF5, Q9BX92, B0QZQ6, |
| UniProt Related Accession: | P00751 |
| Molecular Weight: | 68,872 Da |
| NCBI Full Name: | Complement factor B |
| NCBI Synonym Full Names: | complement factor B |
| NCBI Official Symbol: | CFB |
| NCBI Official Synonym Symbols: | BF; FB; BFD; GBG; CFAB; CFBD; PBF2; AHUS4; FBI12; H2-Bf; ARMD14 |
| NCBI Protein Information: | complement factor B |
| UniProt Protein Name: | Complement factor B |
| UniProt Synonym Protein Names: | C3/C5 convertase; Glycine-rich beta glycoprotein; GBG |
| Protein Family: | Complement factor |
| UniProt Gene Name: | CFB |
| UniProt Entry Name: | CFAB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Complement Factor B antibody (CAB1706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human mammary cancer using Complement Factor B antibody (CAB1706) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using Complement Factor B antibody (CAB1706) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using Complement Factor B antibody (CAB1706) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of Raw264.7 cells using Complement Factor B Polyclonal Antibody (CAB1706) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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