Anti-CNBP 항체 (CAB15110)

Antibody Name:	Anti-CNBP Antibody
Antibody SKU:	CAB15110
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human CNBP (NP_001120668.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, A-549, LO2, mouse brain, mouse kidney, mouse liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human CNBP (NP_001120668.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LARD CDHA DEQK CYSC GEFG HIQK DCTK VKCY RCGE TGHV AINC SKTS EVNC YRCG ESGH LARE CTIE ATA
Gene ID:	7555
Uniprot:	P62633
Cellular Location:	Cytoplasm, Endoplasmic reticulum
Calculated MW:	17kDa/18kDa/19kDa
Observed MW:	19kDa

Synonyms:

CNBP, CNBP1, DM2, PROMM, RNF163, ZCCHC22, ZNF9

Background:

This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion from <30 repeats to 75-11000 repeats in the first intron of this gene results in myotonic dystrophy type 2. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ZNF9: Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression. Defects in CNBP are the cause of dystrophia myotonica type 2 (DM2); also known as proximal myotonic myopathy (PROMM). A multisystem disease characterized by the association of proximal muscle weakness with myotonia, cardiac manifestations and cataract. Additional features can include hyperhidrosis, testicular atrophy, insulin resistance and diabetes and central nervous system anomalies in rare cases. The causative mutation is a CCTG expansion (mean approximately 5000 repeats) located in intron 1 of the CNBP gene. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 3q21 Cellular Component: cytosol; endoplasmic reticulum; nucleus Molecular Function:protein binding; transcription factor activity Biological Process: cholesterol biosynthetic process; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent Disease: Myotonic Dystrophy 2
NCBI Summary:	This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion from <30 repeats to 75-11000 repeats in the first intron of this gene results in myotonic dystrophy type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2016]
UniProt Code:	P62633
NCBI GenInfo Identifier:	50401852
NCBI Gene ID:	7555
NCBI Accession:	P62633.1
UniProt Secondary Accession:	P62633,P20694, Q4JGY0, Q4JGY1, Q5QJR0, A8K7V4, B2RAV9 B4DP17, D3DNB9, D3DNC0, D3DNC1, E9PDR7,
UniProt Related Accession:	P62633
Molecular Weight:	18,871 Da
NCBI Full Name:	Cellular nucleic acid-binding protein
NCBI Synonym Full Names:	CCHC-type zinc finger nucleic acid binding protein
NCBI Official Symbol:	CNBP
NCBI Official Synonym Symbols:	DM2; ZNF9; CNBP1; PROMM; RNF163; ZCCHC22
NCBI Protein Information:	cellular nucleic acid-binding protein
UniProt Protein Name:	Cellular nucleic acid-binding protein
UniProt Synonym Protein Names:	Zinc finger protein 9
Protein Family:	Cellular nucleic acid-binding protein
UniProt Gene Name:	CNBP
UniProt Entry Name:	CNBP_HUMAN

	Western blot analysis of extracts of various cell lines, using CNBP antibody (CAB15110) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of U-2 OS cells using CNBP antibody (CAB15110) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

CNBP Antibody (PACO08480)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)