Anti-CKB Antibody (CAB12632)
- SKU:
- CAB12632
- Product type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-CKB Antibody |
| Antibody SKU: | CAB12632 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 182-381 of human CKB (NP_001814.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:3000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse brain, Mouse liver, Rat spinal cord |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 182-381 of human CKB (NP_001814.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AEQQ QLID DHFL FDKP VSPL LLAS GMAR DWPD ARGI WHND NKTF LVWV NEED HLRV ISMQ KGGN MKEV FTRF CTGL TQIE TLFK SKDY EFMW NPHL GYIL TCPS NLGT GLRA GVHI KLPN LGKH EKFS EVLK RLRL QKRG TGGV DTAA VGGV FDVS NADR LGFS EVEL VQMV VDGV KLLI EMEQ RLEQ GQAI DDLM PAQK |
| Gene ID: | 1152 |
| Uniprot: | P12277 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 42kDa |
| Observed MW: | 43kDa |
| Synonyms: | CKB, B-CK, BCK, CKBB, HEL-211, HEL-S-29 |
| Background: | The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. |
| UniProt Protein Function: | Function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. |
| UniProt Protein Details: | Catalytic activity: ATP + creatine = ADP + phosphocreatine. Subunit structure: Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Subcellular location: Cytoplasm. Sequence similarities: Belongs to the ATP:guanido phosphotransferase family.Contains 1 phosphagen kinase C-terminal domain.Contains 1 phosphagen kinase N-terminal domain. |
| NCBI Summary: | The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq] |
| UniProt Code: | P12277 |
| NCBI GenInfo Identifier: | 125294 |
| NCBI Gene ID: | 1152 |
| NCBI Accession: | P12277.1 |
| UniProt Secondary Accession: | P12277,Q2LE07, Q6FG40, Q9UC66, A8K236, B2R5R4, |
| UniProt Related Accession: | P12277 |
| Molecular Weight: | 42,644 Da |
| NCBI Full Name: | Creatine kinase B-type |
| NCBI Synonym Full Names: | creatine kinase, brain |
| NCBI Official Symbol: | CKB |
| NCBI Official Synonym Symbols: | B-CK; CKBB |
| NCBI Protein Information: | creatine kinase B-type; creatine kinase-B; creatine kinase B chain |
| UniProt Protein Name: | Creatine kinase B-type |
| UniProt Synonym Protein Names: | B-CK; Creatine kinase B chain |
| Protein Family: | Creatine kinase |
| UniProt Gene Name: | CKB |
| UniProt Entry Name: | KCRB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CKB antibody (CAB12632) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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