Anti-CISH Antibody (CAB6286)
- SKU:
- CAB6286
- Product type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-CISH Antibody |
| Antibody SKU: | CAB6286 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-257 of human CISH (NP_659508.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-257 of human CISH (NP_659508.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MVLC VQGP RPLL AVER TGQR PLWA PSLE LPKP VMQP LPAG AFLE EVAE GTPA QTES EPKV LDPE EDLL CIAK TFSY LRES GWYW GSIT ASEA RQHL QKMP EGTF LVRD STHP SYLF TLSV KTTR GPTN VRIE YADS SFRL DSNC LSRP RILA FPDV VSLV QHYV ASCT ADTR SDSP DPAP TPAL PMPK EDAP SDPA LPAP PPAT AVHL KLVQ PFVR RSSA RSLQ HLCR LVIN RLVA DVDC LPLP RRMA DYLR QYPF Q |
| Gene ID: | 1154 |
| Uniprot: | Q9NSE2 |
| Cellular Location: | |
| Calculated MW: | 28kDa/29kDa/30kDa |
| Observed MW: | 37kDa |
| Synonyms: | CISH, BACTS2, CIS, CIS-1, G18, SOCS |
| Background: | The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | CISH: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate- recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Stably associated with the tyrosine-phosphorylated IL3 receptor beta chain and tyrosine-phosphorylated EPO receptor (EPOR). By a subset of cytokines including EPO/erythropoietin. Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 3p21.3 Cellular Component: cytoplasm; plasma membrane; cytosol Molecular Function:protein binding; protein kinase inhibitor activity Biological Process: cytokine and chemokine mediated signaling pathway; protein kinase C activation; negative regulation of insulin receptor signaling pathway; negative regulation of protein kinase activity; protein ubiquitination; regulation of cell growth; negative regulation of JAK-STAT cascade Disease: Mycobacterium Tuberculosis, Susceptibility To; Bacteremia, Susceptibility To, 2; Malaria, Susceptibility To |
| NCBI Summary: | The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| UniProt Code: | Q9NSE2 |
| NCBI GenInfo Identifier: | 13124022 |
| NCBI Gene ID: | 1154 |
| NCBI Accession: | Q9NSE2.1 |
| UniProt Secondary Accession: | Q9NSE2,Q9NS38, Q9Y5R1, B2R9N1, G5E9R1, |
| UniProt Related Accession: | Q9NSE2 |
| Molecular Weight: | 30,734 Da |
| NCBI Full Name: | Cytokine-inducible SH2-containing protein |
| NCBI Synonym Full Names: | cytokine inducible SH2-containing protein |
| NCBI Official Symbol: | CISH |
| NCBI Official Synonym Symbols: | CIS; G18; SOCS; CIS-1; BACTS2 |
| NCBI Protein Information: | cytokine-inducible SH2-containing protein; suppressor of cytokine signaling; cytokine-inducible inhibitor of signaling type 1B |
| UniProt Protein Name: | Cytokine-inducible SH2-containing protein |
| UniProt Synonym Protein Names: | CIS-1; Protein G18; Suppressor of cytokine signaling; SOCS |
| Protein Family: | Cis-abienol synthase |
| UniProt Gene Name: | CISH |
| UniProt Entry Name: | CISH_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CISH antibody (CAB6286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
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