Anti-CISH Antibody (CAB14527)
- SKU:
- CAB14527
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-CISH Antibody |
| Antibody SKU: | CAB14527 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 17-130 of human CISH (NP_659508.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 17-130 of human CISH (NP_659508.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TGQR PLWA PSLE LPKP VMQP LPAG AFLE EVAE GTPA QTES EPKV LDPE EDLL CIAK TFSY LRES GWYW GSIT ASEA RQHL QKMP EGTF LVRD STHP SYLF TLSV KTTR GPTN VR |
| Gene ID: | 1154 |
| Uniprot: | Q9NSE2 |
| Cellular Location: | |
| Calculated MW: | 28kDa/29kDa/30kDa |
| Observed MW: | 37kDa |
| Synonyms: | CISH, BACTS2, CIS, CIS-1, G18, SOCS |
| Background: | The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | CISH: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate- recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Stably associated with the tyrosine-phosphorylated IL3 receptor beta chain and tyrosine-phosphorylated EPO receptor (EPOR). By a subset of cytokines including EPO/erythropoietin. Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 3p21.3 Cellular Component: cytoplasm; plasma membrane; cytosol Molecular Function:protein binding; protein kinase inhibitor activity Biological Process: cytokine and chemokine mediated signaling pathway; protein kinase C activation; negative regulation of insulin receptor signaling pathway; negative regulation of protein kinase activity; protein ubiquitination; regulation of cell growth; negative regulation of JAK-STAT cascade Disease: Mycobacterium Tuberculosis, Susceptibility To; Bacteremia, Susceptibility To, 2; Malaria, Susceptibility To |
| NCBI Summary: | The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| UniProt Code: | Q9NSE2 |
| NCBI GenInfo Identifier: | 13124022 |
| NCBI Gene ID: | 1154 |
| NCBI Accession: | Q9NSE2.1 |
| UniProt Secondary Accession: | Q9NSE2,Q9NS38, Q9Y5R1, B2R9N1, G5E9R1, |
| UniProt Related Accession: | Q9NSE2 |
| Molecular Weight: | 30,734 Da |
| NCBI Full Name: | Cytokine-inducible SH2-containing protein |
| NCBI Synonym Full Names: | cytokine inducible SH2-containing protein |
| NCBI Official Symbol: | CISH |
| NCBI Official Synonym Symbols: | CIS; G18; SOCS; CIS-1; BACTS2 |
| NCBI Protein Information: | cytokine-inducible SH2-containing protein; suppressor of cytokine signaling; cytokine-inducible inhibitor of signaling type 1B |
| UniProt Protein Name: | Cytokine-inducible SH2-containing protein |
| UniProt Synonym Protein Names: | CIS-1; Protein G18; Suppressor of cytokine signaling; SOCS |
| Protein Family: | Cis-abienol synthase |
| UniProt Gene Name: | CISH |
| UniProt Entry Name: | CISH_HUMAN |
 | Western blot analysis of extracts of 293T cells, using CISH antibody (CAB14527) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunofluorescence analysis of C6 cells using CISH antibody (CAB14527) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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