Anti-cIAP2 Antibody (CAB0833)
- SKU:
- CAB0833
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-cIAP2 Antibody |
| Antibody SKU: | CAB0833 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human cIAP2 (NP_001156.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, RAW264.7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human cIAP2 (NP_001156.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MNIV ENSI FLSN LMKS ANTF ELKY DLSC ELYR MSTY STFP AGVP VSER SLAR AGFY YTGV NDKV KCFC CGLM LDNW KRGD SPTE KHKK LYPS CRFV QSLN SVNN LEAT SQPT FPSS VTNS THSL LPGT ENSG YFRG SYSN SPSN PVNS RANQ DFSA LMRS SYHC AMNN ENAR LLTF QTWP |
| Gene ID: | 330 |
| Uniprot: | Q13489 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 68kDa |
| Observed MW: | 75KDa |
| Synonyms: | BIRC3, AIP1, API2, CIAP2, HAIP1, HIAP1, MALT2, MIHC, RNF49, c-IAP2 |
| Background: | This gene encodes a member of the IAP family of proteins that inhibit apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. It contains 3 baculovirus IAP repeats and a ring finger domain. Transcript variants encoding the same isoform have been identified. |
| UniProt Protein Function: | cIAP2: Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin- protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin- protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase- independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Interacts with DIABLO/SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2). Interacts with RIP1, RIP2, RIP3, RIP4 and USP19. Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes. USP19 regulates the stability of BIRC3/c-IAP2 by preventing its ubiquitination. Belongs to the IAP family. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase; EC 6.3.2.-; Ligase; Apoptosis; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 11q22 Cellular Component: nucleoplasm; spindle microtubule; cytoplasm; nucleus; cytosol; lipid raft Molecular Function:protein binding; zinc ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; ligase activity Biological Process: regulation of toll-like receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein heterooligomerization; MyD88-independent toll-like receptor signaling pathway; protein ubiquitination; toll-like receptor 3 signaling pathway; regulation of innate immune response; regulation of apoptosis; cell surface receptor linked signal transduction; positive regulation of protein ubiquitination; regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; negative regulation of phosphorylation; spermatogenesis; toll-like receptor 4 signaling pathway; negative regulation of apoptosis |
| NCBI Summary: | This gene encodes a member of the IAP family of proteins that inhibit apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. It contains 3 baculovirus IAP repeats and a ring finger domain. Transcript variants encoding the same isoform have been identified. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q13489 |
| NCBI GenInfo Identifier: | 2497236 |
| NCBI Gene ID: | 330 |
| NCBI Accession: | Q13489.2 |
| UniProt Related Accession: | Q13489 |
| Molecular Weight: | 72kDa |
| NCBI Full Name: | Baculoviral IAP repeat-containing protein 3 |
| NCBI Synonym Full Names: | baculoviral IAP repeat containing 3 |
| NCBI Official Symbol: | BIRC3 |
| NCBI Official Synonym Symbols: | AIP1; API2; MIHC; CIAP2; HAIP1; HIAP1; IAP-1; MALT2; RNF49; c-IAP2 |
| NCBI Protein Information: | baculoviral IAP repeat-containing protein 3 |
| UniProt Protein Name: | Baculoviral IAP repeat-containing protein 3 |
| UniProt Synonym Protein Names: | Apoptosis inhibitor 2; API2; C-IAP2; IAP homolog C; Inhibitor of apoptosis protein 1; IAP-1; hIAP-1; hIAP1; RING finger protein 49; TNFR2-TRAF-signaling complex protein 1 |
| Protein Family: | Probable apoptosis inhibitor |
| UniProt Gene Name: | BIRC3 |
| UniProt Entry Name: | BIRC3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using cIAP2 antibody (CAB0833) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using cIAP2 Rabbit pAb (CAB0833) at dilution of 1:50 (40x lens). |
 | Immunofluorescence analysis of C6 cells using cIAP2 Rabbit pAb (CAB0833) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using cIAP2 Rabbit pAb (CAB0833) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. |
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