Anti-CHST2 Antibody (CAB15755)
- SKU:
- CAB15755
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-CHST2 Antibody |
| Antibody SKU: | CAB15755 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 231-530 of human CHST2 (NP_004258.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | U-251MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 231-530 of human CHST2 (NP_004258.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FQLY SPAG SGGR NLTT LGIF GAAT NKVV CSSP LCPA YRKE VVGL VDDR VCKK CPPQ RLAR FEEE CRKY RTLV IKGV RVFD VAVL APLL RDPA LDLK VIHL VRDP RAVA SSRI RSRH GLIR ESLQ VVRS RDPR AHRM PFLE AAGH KLGA KKEG VGGP ADYH ALGA MEVI CNSM AKTL QTAL QPPD WLQG HYLV VRYE DLVG DPVK TLRR VYDF VGLL VSPE MEQF ALNM TSGS GSSS KPFV VSAR NATQ AANA WRTA LTFQ QIKQ VEEF CYQP MAVL GYER VNSP EEVK DLSK TLLR KPRL |
| Gene ID: | 9435 |
| Uniprot: | Q9Y4C5 |
| Cellular Location: | Golgi apparatus, Single-pass type II membrane protein, trans-Golgi network membrane |
| Calculated MW: | 52kDa/57kDa |
| Observed MW: | 60kDa |
| Synonyms: | CHST2, C6ST, GST-2, GST2, Gn6ST-1, HEL-S-75, glcNAc6ST-1 |
| Background: | This locus encodes a sulfotransferase protein. The encoded enzyme catalyzes the sulfation of a nonreducing N-acetylglucosamine residue, and may play a role in biosynthesis of 6-sulfosialyl Lewis X antigen. |
| UniProt Protein Function: | CHST2: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. 2 isoforms of the human protein are produced by alternative initiation. |
| UniProt Protein Details: | Protein type:EC 2.8.2.-; Membrane protein, integral; Transferase; Glycan Metabolism - keratan sulfate biosynthesis Chromosomal Location of Human Ortholog: 3q24 Cellular Component: Golgi membrane; integral to membrane; trans-Golgi network Molecular Function:N-acetylglucosamine 6-O-sulfotransferase activity; sulfotransferase activity Biological Process: keratan sulfate biosynthetic process; multicellular organismal development; N-acetylglucosamine metabolic process; sulfur metabolic process |
| NCBI Summary: | This locus encodes a sulfotransferase protein. The encoded enzyme catalyzes the sulfation of a nonreducing N-acetylglucosamine residue, and may play a role in biosynthesis of 6-sulfosialyl Lewis X antigen. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q9Y4C5 |
| NCBI GenInfo Identifier: | 61212252 |
| NCBI Gene ID: | 9435 |
| NCBI Accession: | Q9Y4C5.2 |
| UniProt Secondary Accession: | Q9Y4C5,Q2M370, Q9GZN5, Q9UED5, Q9Y6F2, D3DNG5, |
| UniProt Related Accession: | Q9Y4C5 |
| Molecular Weight: | 52,787 Da |
| NCBI Full Name: | Carbohydrate sulfotransferase 2 |
| NCBI Synonym Full Names: | carbohydrate sulfotransferase 2 |
| NCBI Official Symbol: | CHST2 |
| NCBI Official Synonym Symbols: | C6ST; GST2; GST-2; Gn6ST-1; HEL-S-75; glcNAc6ST-1 |
| NCBI Protein Information: | carbohydrate sulfotransferase 2 |
| UniProt Protein Name: | Carbohydrate sulfotransferase 2 |
| UniProt Synonym Protein Names: | Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2; GST-2; N-acetylglucosamine 6-O-sulfotransferase 1; GlcNAc6ST-1; Gn6ST-1 |
| Protein Family: | Carbohydrate sulfotransferase |
| UniProt Gene Name: | CHST2 |
| UniProt Entry Name: | CHST2_HUMAN |
 | Western blot analysis of extracts of U-251MG cells, using CHST2 antibody (CAB15755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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