Anti-CHRDL1 항체 (CAB15204)

Antibody Name:	Anti-CHRDL1 Antibody
Antibody SKU:	CAB15204
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 289-458 of human CHRDL1 (NP_001137453.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HeLa, mouse heart, mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 289-458 of human CHRDL1 (NP_001137453.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CTCN VTKQ ECKK IHCP NRYP CKYP QKID GKCC KVCP GKKA KEEL PGQS FDNK GYFC GEET MPVY ESVF MEDG ETTR KIAL ETER PPQV EVHV WTIR KGIL QHFH IEKI SKRM FEEL PHFK LVTR TTLS QWKI FTEG EAQI SQMC SSRV CRTE LEDL VKVL YLER SEKG HC
Gene ID:	91851
Uniprot:	Q9BU40
Cellular Location:	Secreted
Calculated MW:	43kDa/51kDa/52kDa
Observed MW:	51kDa

Synonyms:	CHRDL1, CHL, MGC1, MGCN, NRLN1, VOPT, dA141H5.1
Background:	This gene encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described.

UniProt Protein Function:	CHRDL1: Antagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation. May also play an important role in regulating retinal angiogenesis through modulation of BMP4 actions in endothelial cells. Plays a role during anterior segment eye development. Defects in CHRDL1 are the cause of megalocornea type 1, X-linked (MGC1). MGC1 is an eye disorder in which the corneal diameter is bilaterally enlarged (greater than 13 mm) without an increase in intraocular pressure. It may also be referred to as anterior megalophthalmos, since the entire anterior segment is larger than normal. Features of megalocornea in addition to a deep anterior chamber include astigmatic refractive errors, atrophy of the iris stroma, miosis secondary to decreased function of the dilator muscle, iridodonesis, and tremulousness, subluxation, or dislocation of the lens. Whereas most affected individuals exhibit normal ocular function, complications include cataract development and glaucoma following lenticular dislocation or subluxation. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: Xq23 Cellular Component: extracellular region Biological Process: BMP signaling pathway; compound eye development; eye development Disease: Megalocornea
NCBI Summary:	This gene encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]
UniProt Code:	Q9BU40
NCBI GenInfo Identifier:	27805644
NCBI Gene ID:	91851
NCBI Accession:	Q9BU40.1
UniProt Secondary Accession:	Q9BU40,Q539E4, Q9Y3H7, B1AKD0, B4DMP3, D3DUY6, E9PGS5
UniProt Related Accession:	Q9BU40
Molecular Weight:	52,026 Da
NCBI Full Name:	Chordin-like protein 1
NCBI Synonym Full Names:	chordin like 1
NCBI Official Symbol:	CHRDL1
NCBI Official Synonym Symbols:	CHL; MGC1; MGCN; VOPT; NRLN1; dA141H5.1
NCBI Protein Information:	chordin-like protein 1
UniProt Protein Name:	Chordin-like protein 1
UniProt Synonym Protein Names:	Neuralin-1; Neurogenesin-1; Ventroptin
Protein Family:	Chordin-like protein
UniProt Gene Name:	CHRDL1
UniProt Entry Name:	CRDL1_HUMAN

Western blot analysis of extracts of various cell lines, using CHRDL1 antibody (CAB15204) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)