Anti-CHD3 항체 (CAB2221)

Antibody Name:	Anti-CHD3 Antibody
Antibody SKU:	CAB2221
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1900-2000 of human CHD3 (NP_001005273.1).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:1000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, Mouse brain, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1900-2000 of human CHD3 (NP_001005273.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ERSI LSRL ASKG TEPH PTPA YPPG PYAT PPGY GAAF SAAP VGAL AAAG ANYS QMPA GSFI TAAT NGPP VLVK KEKE MVGA LVSD GLDR KEPR AGEV ICID D
Gene ID:	1107
Uniprot:	Q12873
Cellular Location:	Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	222kDa/226kDa/233kDa
Observed MW:	280kDa

Synonyms:

CHD3, Mi-2a, Mi2-ALPHA, ZFH

Background:

This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.

UniProt Protein Function:	Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
NCBI Summary:	This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	Q12873
NCBI GenInfo Identifier:	88911273
NCBI Gene ID:	1107
NCBI Accession:	Q12873.3
UniProt Secondary Accession:	Q12873,Q9Y4I0, D3DTQ9, E9PG89,
UniProt Related Accession:	Q12873
Molecular Weight:	233,037 Da
NCBI Full Name:	Chromodomain-helicase-DNA-binding protein 3
NCBI Synonym Full Names:	chromodomain helicase DNA binding protein 3
NCBI Official Symbol:	CHD3
NCBI Official Synonym Symbols:	ZFH; Mi-2a; Mi2-ALPHA
NCBI Protein Information:	chromodomain-helicase-DNA-binding protein 3
UniProt Protein Name:	Chromodomain-helicase-DNA-binding protein 3
UniProt Synonym Protein Names:	ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Mi2-alpha; Zinc finger helicase; hZFH
Protein Family:	CHD3-type chromatin-remodeling factor
UniProt Gene Name:	CHD3
UniProt Entry Name:	CHD3_HUMAN

Western blot analysis of extracts of various cell lines, using CHD3 antibody (CAB2221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.

ELISA Kits

Human CHD3 (Chromodomain Helicase DNA Binding Protein 3) ELISA Kit (HUES01882)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)