Anti-CHAT Antibody (CAB16818)
- SKU:
- CAB16818
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Neuroscience
Frequently bought together:
Description
| Antibody Name: | Anti-CHAT Antibody |
| Antibody SKU: | CAB16818 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 331-630 of human CHAT (NP_001136401.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Raji, U-87MG, HepG2, HeLa, C6 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 331-630 of human CHAT (NP_001136401.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VLVQ CTEH LLKH MTQS SRKL IRAD SVSE LPAP RRLR WKCS PEIQ GHLA SSAE KLQR IVKN LDFI VYKF DNYG KTFI KKQK CSPD AFIQ VALQ LAFY RLHR RLVP TYES ASIR RFQE GRVD NIRS ATPE ALAF VRAV TDHK AAVP ASEK LLLL KDAI RAQT AYTV MAIT GMAI DNHL LALR ELAR AMCK ELPE MFMD ETYL MSNR FVLS TSQV PTTT EMFC CYGP VVPN GYGA CYNP QPET ILFC ISSF HSCK ETSS SKFA KAVE ESLI DMRD LCSL LPPT ESKP LATK EKAT RPSQ GHQP |
| Gene ID: | 1103 |
| Uniprot: | P28329 |
| Cellular Location: | |
| Calculated MW: | 70kDa/74kDa/82kDa |
| Observed MW: | 70kDa |
| Synonyms: | CHAT, CHOACTASE, CMS1A, CMS1A2, CMS6 |
| Background: | This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. |
| UniProt Protein Function: | CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described. |
| UniProt Protein Details: | Protein type:EC 2.3.1.6; Lipid Metabolism - glycerophospholipid; Acetyltransferase Chromosomal Location of Human Ortholog: 10q11.2 Cellular Component: cell soma; mitochondrion; axon; cytoplasm; nucleus; cytosol Molecular Function:choline O-acetyltransferase activity Biological Process: rhythmic excitation; muscle development; neurotransmitter secretion; glycerophospholipid biosynthetic process; adult walking behavior; synaptic transmission; rhythmic behavior; phospholipid metabolic process; phosphatidylcholine biosynthetic process; dendrite development; neuromuscular synaptic transmission; establishment of synaptic specificity at neuromuscular junction; neurotransmitter biosynthetic process Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea |
| NCBI Summary: | This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010] |
| UniProt Code: | P28329 |
| NCBI GenInfo Identifier: | 281185509 |
| NCBI Gene ID: | 1103 |
| NCBI Accession: | P28329.4 |
| UniProt Secondary Accession: | P28329,Q16488, Q9BQ23, Q9BQ35, Q9BQE1, A2BDF4, A2BDF5 |
| UniProt Related Accession: | P28329 |
| Molecular Weight: | 748 |
| NCBI Full Name: | Choline O-acetyltransferase |
| NCBI Synonym Full Names: | choline O-acetyltransferase |
| NCBI Official Symbol: | CHAT |
| NCBI Official Synonym Symbols: | CMS1A; CMS1A2; CHOACTASE |
| NCBI Protein Information: | choline O-acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase |
| UniProt Protein Name: | Choline O-acetyltransferase |
| Protein Family: | (Z)-3-hexen-1-ol acetyltransferase |
| UniProt Gene Name: | CHAT |
| UniProt Entry Name: | CLAT_HUMAN |
 | Western blot analysis of extracts of various cell line, using CHAT antibody (CAB16818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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