Anti-CHAMP1 Antibody (CAB11690)
- SKU:
- CAB11690
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-CHAMP1 Antibody |
| Antibody SKU: | CAB11690 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 563-812 of human CHAMP1 (NP_115812.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-87MG, A-431, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 563-812 of human CHAMP1 (NP_115812.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LPKS ALFS ESQK AVEL GDEL QIDA IDDQ KCDI LVQE ELLA SPKK LLED TLFP SSKK LKKD NQES SDAE LSSS EYIK TDLD AMDI KGQE SSSD QEQV DVES IDFS KENK MDMT SPEQ SRNV LQFT EEKE AFIS EEEI AKYM KRGK GKYY CKIC CCRA MKKG AVLH HLVN KHNV HSPY KCTI CGKA FLLE SLLK NHVA AHGQ SLLK CPRC NFES NFPR GFKK HLTH CQSR HNEE ANKK LMEA LEPP LEEQ QI |
| Gene ID: | 283489 |
| Uniprot: | Q96JM3 |
| Cellular Location: | Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, kinetochore, spindle |
| Calculated MW: | 89kDa |
| Observed MW: | 130kDa |
| Synonyms: | CHAMP1, C13orf8, CAMP, CHAMP, MRD40, ZNF828 |
| Background: |
| UniProt Protein Function: | ZNF828: Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore. |
| UniProt Protein Details: | Protein type:C2H2-type zinc finger protein; RNA-binding Chromosomal Location of Human Ortholog: 13q34 Cellular Component: condensed chromosome; nuclear body; nucleoplasm; nucleus; spindle Molecular Function:protein binding Biological Process: attachment of spindle microtubules to kinetochore during mitosis; sister chromatid biorientation Disease: Mental Retardation, Autosomal Dominant 40 |
| NCBI Summary: | This gene encodes a zinc finger protein that functions as a regulator of chromosome segregation in mitosis. The encoded protein is required for correct alignment of chromosomes on the metaphase plate, and plays a role in maintaining the attachment of sister kinetochores to microtubules from opposite spindle poles. Mutations in this gene are associated with autosomal dominant mental retardation-40. [provided by RefSeq, Oct 2016] |
| UniProt Code: | Q96JM3 |
| NCBI GenInfo Identifier: | 114149935 |
| NCBI Gene ID: | 283489 |
| NCBI Accession: | Q96JM3.2 |
| UniProt Secondary Accession: | Q96JM3,Q6P181, Q8NC88, Q9BST0, B3KU06, |
| UniProt Related Accession: | Q96JM3 |
| Molecular Weight: | 89kDa |
| NCBI Full Name: | Chromosome alignment-maintaining phosphoprotein 1 |
| NCBI Synonym Full Names: | chromosome alignment maintaining phosphoprotein 1 |
| NCBI Official Symbol: | CHAMP1 |
| NCBI Official Synonym Symbols: | CAMP; CHAMP; MRD40; ZNF828; C13orf8 |
| NCBI Protein Information: | chromosome alignment-maintaining phosphoprotein 1 |
| UniProt Protein Name: | Chromosome alignment-maintaining phosphoprotein 1 |
| UniProt Synonym Protein Names: | Zinc finger protein 828 |
| Protein Family: | Chromosome alignment-maintaining phosphoprotein |
| UniProt Gene Name: | CHAMP1 |
 | Western blot analysis of extracts of various cell lines, using CHAMP1 Antibody (CAB11690) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of Hela cells using CHAMP1 antibody (CAB11690) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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