Anti-cFLIP Antibody (CAB2555)
- SKU:
- CAB2555
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-cFLIP Antibody |
| Antibody SKU: | CAB2555 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 161-480 of human cFLIP (NP_001120655.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, Mouse liver, Mouse heart, Rat liver, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 161-480 of human cFLIP (NP_001120655.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RIDL KTKI QKYK QSVQ GAGT SYRN VLQA AIQK SLKD PSNN FRLH NGRS KEQR LKEQ LGAQ QEPV KKSI QESE AFLP QSIP EERY KMKS KPLG ICLI IDCI GNET ELLR DTFT SLGY EVQK FLHL SMHG ISQI LGQF ACMP EHRD YDSF VCVL VSRG GSQS VYGV DQTH SGLP LHHI RRMF MGDS CPYL AGKP KMFF IQNY VVSE GQLE DSSL LEVD GPAM KNVE FKAQ KRGL CTVH READ FFWS LCTA DMSL LEQS HSSP SLYL QCLS QKLR QERK RPLL DLHI ELNG YMYD WNSR VSAK EKYY VWLQ HTLR KKLI LSYT |
| Gene ID: | 8837 |
| Uniprot: | O15519 |
| Cellular Location: | |
| Calculated MW: | 23-55kDa |
| Observed MW: | 28kDa/55kDa |
| Synonyms: | CFLAR, CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS |
| Background: | The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. |
| NCBI Summary: | The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011] |
| UniProt Code: | O15519 |
| NCBI GenInfo Identifier: | 12643547 |
| NCBI Gene ID: | 8837 |
| NCBI Accession: | O15519.1 |
| UniProt Related Accession: | O15519 |
| Molecular Weight: | ~ 55kDa |
| NCBI Full Name: | CASP8 and FADD-like apoptosis regulator |
| NCBI Synonym Full Names: | CASP8 and FADD like apoptosis regulator |
| NCBI Official Symbol: | CFLAR |
| NCBI Official Synonym Symbols: | CASH; FLIP; MRIT; CLARP; FLAME; cFLIP; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1 |
| NCBI Protein Information: | CASP8 and FADD-like apoptosis regulator |
| UniProt Protein Name: | CASP8 and FADD-like apoptosis regulator |
| UniProt Synonym Protein Names: | Caspase homolog; CASH; Caspase-eight-related protein; Casper; Caspase-like apoptosis regulatory protein; CLARP; Cellular FLICE-like inhibitory protein; c-FLIP; FADD-like antiapoptotic molecule 1; FLAME-1; Inhibitor of FLICE; I-FLICE; MACH-related inducer of toxicity; MRIT; UsurpinCleaved into the following 2 chains:CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12 |
| Protein Family: | Flippase kinase |
| UniProt Gene Name: | CFLAR |
| UniProt Entry Name: | CFLAR_HUMAN |
 | Western blot analysis of extracts of Jurkat cells, using cFLIP antibody (CAB2555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of extracts of various cell lines, using cFLIP antibody (CAB2555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human thyroid cancer using cFLIP antibody (CAB2555) at dilution of 1:100 (40x lens). |
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