Anti-CENPC Antibody (CAB6557)
- SKU:
- CAB6557
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-CENPC Antibody |
| Antibody SKU: | CAB6557 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CENPC (NP_001803.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, A-431, HepG2, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CENPC (NP_001803.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAS GLDH LKNG YRRR FCRP SRAR DINT EQGQ NVLE ILQD CFEE KSLA NDFS TNST KSVP NSTR KIKD TCIQ SPSK ECQK SHPK SVPV SSKK KEAS LQFV VEPS EATN RSVQ AHEV HQKI LATD VSSK NTPD SKKI SSRN INDH HSEA DEEF YLSV GSPS VLLD AKTS VSQN VIPS SAQK RETY TFEN SVNM LPSS TEVS VKTK KRLN FDDK VMLK KIEI DNKV SDEE DKTS EGQE RKPS GSSQ NRIR DSEY EIQR QAKK SFST LFLE TVKR KSES SPIV RHAA TAPP HSCP PDDT KLIE |
| Gene ID: | 1060 |
| Uniprot: | Q03188 |
| Cellular Location: | Chromosome, Nucleus, centromere, kinetochore |
| Calculated MW: | 61kDa/106kDa |
| Observed MW: | 130kDa |
| Synonyms: | CENPC, CENP-C, CENPC1, MIF2, hcp-4 |
| Background: | Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. The protein is required for maintaining proper kinetochore size and a timely transition to anaphase. A putative pseudogene exists on chromosome 12. |
| UniProt Protein Function: | CENPC1: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions. Belongs to the CENPC family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 4q13.2 Cellular Component: centric heterochromatin; condensed nuclear chromosome, pericentric region; cytoplasm; cytosol; kinetochore; nucleoplasm; nucleus Molecular Function:DNA binding Biological Process: chromosome segregation; DNA replication-independent nucleosome assembly at centromere; kinetochore assembly; mitotic cell cycle; sister chromatid cohesion |
| NCBI Summary: | Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. The protein is required for maintaining proper kinetochore size and a timely transition to anaphase. A putative pseudogene exists on chromosome 12. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q03188 |
| NCBI GenInfo Identifier: | 296434446 |
| NCBI Gene ID: | 1060 |
| NCBI Accession: | Q03188.2 |
| UniProt Secondary Accession: | Q03188,Q6PIR0, Q8IW27, Q9P0M5, |
| UniProt Related Accession: | Q03188 |
| Molecular Weight: | 61,544 Da |
| NCBI Full Name: | Centromere protein C |
| NCBI Synonym Full Names: | centromere protein C |
| NCBI Official Symbol: | CENPC |
| NCBI Official Synonym Symbols: | MIF2; hcp-4; CENP-C; CENPC1 |
| NCBI Protein Information: | centromere protein C |
| UniProt Protein Name: | Centromere protein C |
| UniProt Synonym Protein Names: | Centromere autoantigen C; Centromere protein C 1; CENP-C 1; Interphase centromere complex protein 7 |
| Protein Family: | Centromere protein |
| UniProt Gene Name: | CENPC |
| UniProt Entry Name: | CENPC_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CENPC antibody (CAB6557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human oophoroma using CENPC antibody (CAB6557) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human kidney cancer using CENPC antibody (CAB6557) at dilution of 1:200 (40x lens). |
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